Mahatma Gandhi University Botany and Biotechnologyhird semester examination Question paper 2013

University:  Mahatma Gandhi University
Course:  B.Sc
Subject : Botany and Biotechnology third
Year of  Question Paper : 2013


B.Sc. DEGREE (CBCSS) EXAMINATION, JANUARY 2013
Third Semester
B.Sc. Botany and Biotechnology (Double Main)
Core Course—PLANT BIOTECHNOLOGY

Time: Three Hours Maximum Weight : 25
Part A
Answer all questions.
1 Weight for each bunch of four.

Bunch I

1. The chemical used for protoplast fusion:

(a) Polyphenylglyceroi (b) Polyacetylene glysal.

c) Polyethylene glycal. (d) Polyethylene chloride.
2. The aim of anther and pollen culture is to:
a) Produce haploid plants. (b) Produce diploid plants.
(c) Produce mutants. (d) Produce disease resistant plants.
3. Undifferentiated mass of dividing cells in a tissue culture is called:
(a) Embryoid. (b) Callus.
(c) Shield. (d) Aggregate
4. Electrophoration is a technique used with:
(a) Calluses. (b) Pollen.
c) Protoplasts. (d) Cell suspension.
Bunch II
5. Cell suspension cultures require:
(a) Organogenesis. (b) Differentiation
(c) Aggregation. (d) Disaggregation
6. The plant resulting from the fusion of two different protoplasts is called:
(a) Protoplasmic hybrid. (b) Somatic hybrid.
(c) Monohybrid. (d) Dihybrid.
Turn over


2 E730

7. The fastest way to a ripe tomato using tissue culture is by:
(a) Anther pollenculture (b) Plant organ culture.
(c) Protoplast culture (d) Callus culture
8. Chemical used for surface sterilization of plant material in tissue culture:
(a) Ethylacetate (b) Calcium carbonate
c) Mercuric chloride (d) Sodium hydroxide
Bunch III
9. Agrochemical obtained from plant:
a) Pyrethrin
(b) Benzedene.
10. A plant useful in the study of plant genomes because of its small genome size is:

(a) Avena sativa. (b) Petunia porodii
(c) Pisum sativum (d) Arabidopsis thaliana

11. Most plant tissue cultures are initiated from:
(a) Calluses. (b) Explants.
(c) Plantlets (d) Protoplast
12. When the cell wall is enzymatically removed, the isolated protoplast is known as?
a) Naked plant cell. (b) Explant.
(c) Callus. (d) Plantlet
Bunch IV
13. Which of the following is not a method of somatic hybridjzaj0?
(a) Isolation of protoplasts (b) Fusion of nuclei of hetero karyon.
(c) Selection of hybrid. d) Lyophilization
14. The genetic engineering of crop plants can me them healthier and more beneficial to humans:
(a) True.
(b) False.
15. Cybrid is formed by the:
(a) Fusion of two protoplasts
(b)Fusion of cytoplasm of two cells but with one nucleus.
(c) Nucleic acid from different source.
(d) Fusion of nuclei.
16. In merjstem culture, all the plants are genetically identical to the plant from
which the meristem was obtained:
(a) True.
(b) False.
(4 x 1 4)

3 E730
Part B
Answer any five questions.
Weight 1 each.
Write short notes on:
17. Suspension culture.
18. Direct organogenesis.
19. Hardening.
20. Synthetic seed.
24 Cell immobilization.
22. Gene cloning.
23. WTO.
24. Allergenecity.
(5 x 1 = 5)
Part C
Answer any four questions.
Weight 2 each.
25. What is an explant?
26 . What is a callus?
27. What is the significance of somaclonal variations?
28. What is indirect androgenesis?
29. Explain the benefits of transgenics to human health.
30. What are biocolours ? Give examples.
(4 x 2 = 8)
PartD
Answer any two questions.
Weight 4 each.
31. Explain Agrobacterium mediated gene transfer.
32. Write an essay on the implications of intellectual property rights on the
commercialization of biotechnology products.
33. Describe in vitro production of secondary metafolites. (2 x 4 = 8) 

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