Mahatma Gandhi University B.Sc Botany Microbiology third semester examination November 2012 Question paper

University:  Mahatma Gandhi University
Course:  B.Sc Botany
Subject :  Microbiology
Year of  Question Paper : 2012

B.Sc. DEGREE (CBCSS) EXAMINATION, NOVEMBER 2012
Third Semester 
B.Sc. Botany and Biotechnology (Double Main) 
Core Course—MICROBIOLOGY AND MICROBIAL BIOTECHNOLOGY 
Time : Three Hours Maximum Weight: 25 
Part A 
Answer all questions. 
Weight I each for a bunch of four.

Bunch I

1. The image will be darker than the background around it in: 
(a) Light microscopy (b) Fluorescent microscopy. 
(c) Dark field microscopy. (d) TEM. 
2. Peptidoglycan is present in: 
(a) Gram +ve bacteria. (b) Gram —ye bacteria. 
(c) Virus. (d) Fungi. 
3. Sterilization by a course of three periods of bailing at 100°C for 30 minutes at daily intervals is called: 
(a) Tyndallization. (b) Pasteurization. 
(c) Filteration. (d) Radiation. 
4. Pigment bearing membranous structure in photosynthetic bacteria is called: 
(a) Chioroplast. (b) Chromoplast. 
(c) Chromatophores. (d) Leucoplast. 

5. The process of ATP synthesis at the expense of protonmotive force is called: 
(a) Exosmosis. (b) Endosmosis. 
(c) Chemiosmosis. (d) Diffusion. 
6. Hot air oven is used for: 
(a) Dry heat sterilization. (b) Moist heat sterilization. 
(c) Chemical sterilization. (d) Radiation sterilization

7. Movement of bacteria toward or away from chemical stimuli is called: 
(a) Chemotaxis (b) Photo taxis. 
(c) Hydrotaxis. (d) Nastic movement. 
8. The bacterium that carries the prophage is said to be: 
(a) Lysogenic. (b) Pathogenic. 
(c) Virulent. (d) Prophage.

9. Humulin is produced by: 
(a) Bacteria. (b) Fungi. 
(c) Algae. (d) Virus. 
10. Sterilization by steam under pressure is carried out at temperature between: 
(a) 108° C and 147° C.cZ (b) 160° C and 168° C. 
(c) 98° C and 108° C. (d) 1600 C and 190° C. 
11. Which of the following enzyme is thought to be useful for detergent manufacture? 
(a) Amylase. (b) Protease. .../ 
(c) Rennet. (d) Lactase. 
12. Mac Conkeys medium is an example of: 
(a) Differential medium. (b) Indicator medium. 
(c) Selective medium. (d) Enriched medium.

13. Five kingdom classification was proposed by: 
(a) R.H. Wbittaker. (b) T.H. Morgan. 
(c) Jacques Monod. (d) Haeckel. 
14. One of the following is a biopesticide: 
(a) Bacillus thuringjensjs (b) Bacillus megatherjum. 
(c) Rhizobjum. (d) Azotobacter. 
15. Which of the radiation is ionisable? 
(a) Infra-red. (b) Ultra-violet. 
(c) Gamma. (d) Visible. 
16. The fungus used as a weedicjde: 
(a) Phytoththora palmivora. (b) Rhizopus. 
(c) Pythium. (d) Agaricus.(4 x 1= 4)

Part B 
Answer any five questions. 
Weight 4 each.

17. What is glycocalyx? 
18. What is dark field microscopy? 
19. What is the function of cotton plug in Microbiology? 
20. What is infusion broth? 
21. What is spheroplast? 
2 2. Explain the structure and function of flagella. 
23. W'natareprions? 
24. Explain lysogeny.(5 x 1 5)
Part C 
Answer any four questions. 
Weight 2 each.

25. Write notes on biofuels? 
26. Explain microencapsulation techniques. 
2'7. What are the major bacterial antibiotics? 
28. Describe Plackett-Burman design. 
29. Explain the structure of viruses. 
3O. What are antiviral drugs ? Give examples.(4 x 2 = 8)

PartD 
Answer any two questions. 
Weight 4 each.

31. Explain the structure and types of bioreactors.
32. Describe the economic importance of microbes. 
33. What are the different methods to classify bacteria?(2 x 4= 8)

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