Sunday, July 12, 2015

WBUT B.Sc Microbiology MICROBIAL BIOTECHNOLOGY Question Paper 2012

University:West Bengal University of Technology
Course: B.Sc
Subject :  MICROBIAL BIOTECHNOLOGY
Year of  Question Paper : 2012


CS/B.Sc(H), MICROBIO/SEM-4/MBT-404/2012

2012

MICROBIAL BIOTECHNOLOGY

Time Allotted : 3 Hours Full Marks : 70

The figures in the margin indicate full marks.

Candidates are required to give their answers in their own words

as far as practicable.

GROUP – A

( Multiple Choice Type Questions )

1. Choose the correct answers for any ten of the following :

[Marks 10 × 1 = 10]

i) The alga which can produce SCP is

a) Chlorella b) Spirulina

c) Both (a) and (b) d) none of these.

ii) The ideal temperature for biogas production is :

a) 40° C-50° C b) 37° C

c) 30° C-40° C d) 25° C-27° C.

iii) Antibiotics is considered as

a) primary metabolite b) secondary metabolite

c) amino acid d) hormone.

iv) Ethanol can be used as

a) red petrol b) green petrol

c) blue petrol d) brown petrol.

v) Acetic acid is produced by

a) Clostridium botulinum

b) Gluconobacter aceti

c) Lactobacillus acidophilus

d) none of these.vi) The bacteria which degrades toluene is

a) Acinetobacter calcoacetions

b) Achromobacter xylosoxidars

c) Pseudomonas fluorescence

d) Ochrobacterium sp.

vii) The micro-organism which is not an inhabitant of the

biofilm in trickling filter is

a) Alcaligenes b) Ulothrix

c) Spirogyra d) Flavobacterium.

viii) The device which has high BOD removal efficiency is

a) Trickling filter

b) Rotating biological contactor

c) Aerobic ponds

d) Anaerobic ponds.

ix) The source(s) for production of Bio-diesel is/are

a) Jatropa b) Soybean

c) Microalgae d) all of these.

x) The organism(s) responsible for bioleaching of metals

from their respective ores is/are

a) Acidothiobacillus ferroxidans

b) Ferroplasma sp

c) both (a) and (b)

d) Pseudomonas aeruginosa.

xi) Tetracycline was first isolated from

a) Streptomyces gresius

b) Streptomyces aureofaciens

c) Actinomycetes

d) Paecilomyces sp.

xii) Vermicomposting involves conversion of carbon richorganic compounds to

a) sulphur rich compounds

b) nitrogen rich compounds

c) hydrogen rich compounds

d) sulphide rich compounds.

GROUP – B

( Short Answer Type Questions )

Answer any three of the following. [Marks 3 × 5 = 15]

2. What is the biochemistry for microbial leaching of FeS2 ?

Differentiate tank bioleaching from heap bioleaching. [Marks 3 + 2]

3. Why is Mercury considered as a health hazard ?

4. Write in general the production procedure for Antibiotics.

5. How does Pseudomonas putida degrade oil spill ? Name the

plasmid which is responsible for oil spill degradation. [Marks 4 + 1]

6. How does Bacillus thuringiensis work ? What do you mean

by primary and secondary metabolites ? [Marks 3 + 2]

GROUP – C

( Long Answer Type Questions )

Answer any three of the following. [Marks 3 × 15 = 45]

7. What is biofuel ? Classify biofuel. What are the microorganisms

involved in optimum biogas production ? What is

biodiesel and what are the sources from where biodiesel can

be isolated ? Describe in brief the production procedure for

biodiesel production. How can you characterize biodiesel ?

[Marks 2 + 3 + 3 + 5 + 2]

8. Differentiate chemostat from turbidostat. What is a batch

culture ? Draw the growth curve for a typical batch culture.

Derive Monod's equation for continuous culture. Differentiate

batch from continuous culture. What is fed batch culture ?[Marks 3 + 2 + 3 + 4 + 2 + 1]

9. Describe the process of Orleans method for vinegar

production. What is the role of acidification in vinegar

production ? Why acidification is never recommended before

the completion of sugar fermentation ? What is the cause for

darkening of vinegar ? Name the genera for acetic acid

bacteria. Write the reaction for acetic acid production where

oxygen is required. [Marks 5 + 2 + 2 + 2 + 2 + 2]

10. Why is bacterial heavy metal resistance evolved ? What are

metallothionein ? What are the five mechanisms to evade

metal toxicity ? Why is nutrient removal necessary for

tertiary sewage treatment ? [Marks 4 + 2 + 5 + 4]

11. What role is played by amylase in textile industries ? Name

the organisms used for assay of Vitamin B12. How can you

use micro-organisms as pesticides ? Give an example. What

are its advantages over chemical pesticides ? Justify. What is

the purpose and the strategy of biological phosphorus

removal in tertiary treatment of water ? [Marks 2 + 2 + 3 + 4 + 4]

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