University:West Bengal University of Technology
Course: B.Sc Microbiology
Subject : MODEL ORGANISMS IN HUMAN GENOME PROJECT
Year of Question Paper : 2012
CS/B.Sc.(H)BT/MOLBIO/MICROBIO/GENETICS/
SEM-6/MHG-601/2012
2012
MODEL ORGANISMS IN HUMAN GENOME PROJECT
Time Allotted : 3 Hours Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words as
far as practicable.
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following :
10 × 1 = 10
i) Of the Human Genome Project objectives which of the
following is not one of the objectives of the Human
Genome Project ?
a) Create a detailed genetic map of every human
chromosome, with an average of 2–5%
recombination frequency between markers.
b) Obtain a detailed physical map of every human
chromosome, based on overlapping recombinant
DNA molecules cloned as yeast artificial
chromosomes.
c) Clone human beings.
d) Determine the sequence of all expressed human
genes by cDNA cloning and sequencing.
e) Determine the complete DNA sequence of each
human chromosome.ii) Human Genome Project began in
a) 1990 b) 1980
c) 1994 d) 1991.
iii) Microarrays
a) are used for analysis of transcriptomes
b) are made of glass
c) contain RNA sequences
d) contain DNA sequences
e) are smaller than DNA chips.
iv) Which of the following statements are not true ?
a) The yeast genome contains about 6000 genes
b) Proteomes consist of proteins
c) RNA interference is not possible in prokaryotes
d) DNA chips contain oligonucleotides.
v) Whole genome shotgun method was discovered by
a) Celera Genomics b) Nexia Biotechnologies
c) Medarex d) none of these.
vi) Number of autosomes in C. elegans is
a) 5 pairs b) 8 pairs
c) 10 pairs d) none of these.
vii) Medical benefits of HGP include
a) improved diagnosis of diseases
b) gene therapy
c) rational drug design
d) all of these.
viii) Onset of gastrulation in zebra fish is represented by
a) 20% epiboly b) 30% epiboly
c) 50% epiboly d) none of these.ix) Yeast artificial chromosome ( YAC ) can accommodate
foreign DNA inserts of about
a) 100-200 Kb b) 200-500 Kb
c) 500-1000 Kb d) none of these.
x) Total length of repeated sequence in S. cerevisae
genome is about
a) 1 Mb b) 4 Mb
c) 7 Mb d) 13 Mb.
xi) EST stands for
a) Expressing Sequencing Technology
b) Express Sequencing Technology
c) Expressed Sequence Tag
d) none of these.
xii) STR is an example of
a) Microsatellite b) Minisatellite
c) Macrosatellite d) None of these.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following. 3 × 5 = 15
2. Discuss the embryonic development of Danio rario.
3. Discuss the characteristics of the genome of C. elegans.
4. Discuss the essential features of Human genome.
5. Why Arabidopsis has been taken as a model organism ?
Discuss with reasons.
6. What is the basic principle of Sanger dideoxy sequencing ?
GROUP – C
( Long Answer Type Questions )
Answer any three of the following. 3 × 15 = 457. Discuss why Drosophila melanogaster is
regarded as a
model organism. Briefly discuss its life cycle. How is sex
determined in drosophila ? Discuss the characteristics of
Drosophila melanogaster's genome. 3 + 3 + 3 + 6
8. Discuss the methods of reproduction in E. coli. Explain
impacts of E. coli in biotechnogy. Most E. coli strains are
harmless, but some are pathogenic — Explain in the light of
their genome characteristics. Discuss the essential genomic
features of E. coli. 5 + 3 + 3 + 4
9. Discuss the medical and scientific benefits of Human
genome project. 9 + 6
10. Write short notes on any three of the following : 3 × 5
a) Gene annotation
b) Sequence tagged sites
c) RFLP
d) DNA microarray.
11. Why is Arabidopsis thaliana regarded as model organism ?
Discuss the biological features of A. thaliana. Discuss the
essential features of A. thaliana genome. 5 + 5 + 5
========
Course: B.Sc Microbiology
Subject : MODEL ORGANISMS IN HUMAN GENOME PROJECT
Year of Question Paper : 2012
CS/B.Sc.(H)BT/MOLBIO/MICROBIO/GENETICS/
SEM-6/MHG-601/2012
2012
MODEL ORGANISMS IN HUMAN GENOME PROJECT
Time Allotted : 3 Hours Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words as
far as practicable.
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following :
10 × 1 = 10
i) Of the Human Genome Project objectives which of the
following is not one of the objectives of the Human
Genome Project ?
a) Create a detailed genetic map of every human
chromosome, with an average of 2–5%
recombination frequency between markers.
b) Obtain a detailed physical map of every human
chromosome, based on overlapping recombinant
DNA molecules cloned as yeast artificial
chromosomes.
c) Clone human beings.
d) Determine the sequence of all expressed human
genes by cDNA cloning and sequencing.
e) Determine the complete DNA sequence of each
human chromosome.ii) Human Genome Project began in
a) 1990 b) 1980
c) 1994 d) 1991.
iii) Microarrays
a) are used for analysis of transcriptomes
b) are made of glass
c) contain RNA sequences
d) contain DNA sequences
e) are smaller than DNA chips.
iv) Which of the following statements are not true ?
a) The yeast genome contains about 6000 genes
b) Proteomes consist of proteins
c) RNA interference is not possible in prokaryotes
d) DNA chips contain oligonucleotides.
v) Whole genome shotgun method was discovered by
a) Celera Genomics b) Nexia Biotechnologies
c) Medarex d) none of these.
vi) Number of autosomes in C. elegans is
a) 5 pairs b) 8 pairs
c) 10 pairs d) none of these.
vii) Medical benefits of HGP include
a) improved diagnosis of diseases
b) gene therapy
c) rational drug design
d) all of these.
viii) Onset of gastrulation in zebra fish is represented by
a) 20% epiboly b) 30% epiboly
c) 50% epiboly d) none of these.ix) Yeast artificial chromosome ( YAC ) can accommodate
foreign DNA inserts of about
a) 100-200 Kb b) 200-500 Kb
c) 500-1000 Kb d) none of these.
x) Total length of repeated sequence in S. cerevisae
genome is about
a) 1 Mb b) 4 Mb
c) 7 Mb d) 13 Mb.
xi) EST stands for
a) Expressing Sequencing Technology
b) Express Sequencing Technology
c) Expressed Sequence Tag
d) none of these.
xii) STR is an example of
a) Microsatellite b) Minisatellite
c) Macrosatellite d) None of these.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following. 3 × 5 = 15
2. Discuss the embryonic development of Danio rario.
3. Discuss the characteristics of the genome of C. elegans.
4. Discuss the essential features of Human genome.
5. Why Arabidopsis has been taken as a model organism ?
Discuss with reasons.
6. What is the basic principle of Sanger dideoxy sequencing ?
GROUP – C
( Long Answer Type Questions )
Answer any three of the following. 3 × 15 = 457. Discuss why Drosophila melanogaster is
regarded as a
model organism. Briefly discuss its life cycle. How is sex
determined in drosophila ? Discuss the characteristics of
Drosophila melanogaster's genome. 3 + 3 + 3 + 6
8. Discuss the methods of reproduction in E. coli. Explain
impacts of E. coli in biotechnogy. Most E. coli strains are
harmless, but some are pathogenic — Explain in the light of
their genome characteristics. Discuss the essential genomic
features of E. coli. 5 + 3 + 3 + 4
9. Discuss the medical and scientific benefits of Human
genome project. 9 + 6
10. Write short notes on any three of the following : 3 × 5
a) Gene annotation
b) Sequence tagged sites
c) RFLP
d) DNA microarray.
11. Why is Arabidopsis thaliana regarded as model organism ?
Discuss the biological features of A. thaliana. Discuss the
essential features of A. thaliana genome. 5 + 5 + 5
========
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