Solapur University
B.Sc. Biotechnology – I (Semester – II) Examination, 2015
BIOMETRY AND TISSUE CULTURE (Paper – II)
Introduction to Tissue Culture (Old)
Day and Date : Monday, 27-4-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to right indicate full marks.
3) Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 10
1) _________ cells have finite life span on artificial medium.
a) Normal b) Cancerous c) Tumour d) Abnormal
2) _________ of cultured cell is increased by increased attachment of cells to
substrate.
a) Ability b) Efficiency c) Capability d) Activity
3) __________ is used for disaggregation of normal, embryonic as well as
cancerous cell.
a) Cellulase b) Pectinase c) Collagenase d) Trypsin
4) Stomata were more open in plants grown in presence of higher __________
a) Uranium b) Potassium c) Magnesium d) Calcium
5) The __________ of the somatic plant cell of a culture to produce embryoids
is known as embryogenic potential.
a) Capability b) Inability c) Toxicity d) Viability
6) Higher concentration of Auxin exists at the __________
a) Leaves b) Growing tips of plants
c) Xylem and Phloem d) Base of any plant organ
7) Elements required by plants in concentration greater than 0.5 mmol/lit. are
referred as __________
a) Vitamins b) Cofactors
c) Macromolecules d) Micromolecules
SLR-M – 26
8) ___________ is a type of organogenesis by which only adventitious root
formation takes place.
a) Cytogenesis b) Caulogenesis
c) Embryogenesis d) Rhizogenesis
9) __________ method is cheap and quick method of cell separation.
a) Physical b) Clinical c) Enzymatical d) Orbital
10) Viable cells are permeable to ___________
a) Propidium iodide b) Eosin Y.
c) Diacetyl fluoresin d) Fluoresin
2. Answer the following (any five) : 10
1) Culture vessel in PTC.
2) Plant growth hormone.
3) Artificial seed.
4) Function of CO2 incubator.
5) Write a note on organ culture.
6) Write a note secondary cell line.
3. A) Answer the following (any two) : 6
1) Explain membrane integrity assay for viability.
2) Discuss callus culture.
3) Describe oxidative. Browning during micropropagation.
B) Explain in detail role of inorganic micronutrients in plant tissue culture. 4
4. Answer the following (any two) : 10
1) Explain warm trypsinization.
2) Give details of pollen culture.
3) Explain different constituents of serum.
5. Answer the following (any two) : 10
1) Discuss different stages of in vitro clonal propagation.
2) Explain natural media for animal tissue culture.
3) Describe in detail suspension culture.
B.Sc. Biotechnology – I (Semester – II) Examination, 2015
BIOMETRY AND TISSUE CULTURE (Paper – II)
Introduction to Tissue Culture (Old)
Day and Date : Monday, 27-4-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to right indicate full marks.
3) Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 10
1) _________ cells have finite life span on artificial medium.
a) Normal b) Cancerous c) Tumour d) Abnormal
2) _________ of cultured cell is increased by increased attachment of cells to
substrate.
a) Ability b) Efficiency c) Capability d) Activity
3) __________ is used for disaggregation of normal, embryonic as well as
cancerous cell.
a) Cellulase b) Pectinase c) Collagenase d) Trypsin
4) Stomata were more open in plants grown in presence of higher __________
a) Uranium b) Potassium c) Magnesium d) Calcium
5) The __________ of the somatic plant cell of a culture to produce embryoids
is known as embryogenic potential.
a) Capability b) Inability c) Toxicity d) Viability
6) Higher concentration of Auxin exists at the __________
a) Leaves b) Growing tips of plants
c) Xylem and Phloem d) Base of any plant organ
7) Elements required by plants in concentration greater than 0.5 mmol/lit. are
referred as __________
a) Vitamins b) Cofactors
c) Macromolecules d) Micromolecules
SLR-M – 26
8) ___________ is a type of organogenesis by which only adventitious root
formation takes place.
a) Cytogenesis b) Caulogenesis
c) Embryogenesis d) Rhizogenesis
9) __________ method is cheap and quick method of cell separation.
a) Physical b) Clinical c) Enzymatical d) Orbital
10) Viable cells are permeable to ___________
a) Propidium iodide b) Eosin Y.
c) Diacetyl fluoresin d) Fluoresin
2. Answer the following (any five) : 10
1) Culture vessel in PTC.
2) Plant growth hormone.
3) Artificial seed.
4) Function of CO2 incubator.
5) Write a note on organ culture.
6) Write a note secondary cell line.
3. A) Answer the following (any two) : 6
1) Explain membrane integrity assay for viability.
2) Discuss callus culture.
3) Describe oxidative. Browning during micropropagation.
B) Explain in detail role of inorganic micronutrients in plant tissue culture. 4
4. Answer the following (any two) : 10
1) Explain warm trypsinization.
2) Give details of pollen culture.
3) Explain different constituents of serum.
5. Answer the following (any two) : 10
1) Discuss different stages of in vitro clonal propagation.
2) Explain natural media for animal tissue culture.
3) Describe in detail suspension culture.
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