Solapur University BIOTECHNOLOGY Environmental Pollution and Microbial Techniques 2015 Question Papers

Solapur University
B.Sc. I (Semester – II) (New CGPA Pattern) Examination, 2015
BIOTECHNOLOGY
Environmental Pollution and Microbial Techniques
Day and Date : Wednesday, 22-4-2015 Max. Marks : 70
Time : 11.00 a.m. to 2.00 p.m.
N.B. : 1) All questions are compulsory.
2) Draw neat labelled diagrams wherever necessary.
SECTION – I
(Environmental Pollution)
1. Rewrite the following sentences by choosing correct alternatives given below : 5
i) __________ is emitted by internal combustion engine.
a) Oxides of Nitrogen b) CO2
c) PCB d) O3
ii) Heavy metals are toxic and get deposited in ___________
a) Kidney b) Liver c) Bones d) All the above
iii) Radioactive Pollution is caused by ____________
a) Fertilizer industries b) Cement industries
c) Nuclear power plants d) Thermal power plants
iv) Saline Soils can be reclaimed by using _____________
a) Gypsum b) Murate of potash
c) Phosphatic fertilizers d) Organic solvents
v) ___________ is a renewable energy source.
a) Coal b) Petroleum c) Wood d) Solar energy

2. Answer any five of the following : 10
i) Mention any four gases responsible for global warming.
ii) Enlist effects of radiations on humans.
iii) What is BOD ?
iv) Explain effects of pesticides and fertilizers on soil.
v) What is pyrolysis ?
vi) Explain eutrophication.
vii) Enlist effects of Acid Rain.
3. A) Write short notes on any two of the following : 10
i) Write in detail about Bhopal Gas Tragedy.
ii) What is Marine Pollution ? State its effects on marine organisms.
iii) Explain in detail about biogas generation.
B) Answer any one of the following : 10
i) Explain the methods of measurement of radiation and add a note on
Atom bomb.
ii) Explain in detail non-conventional energy sources.
SECTION – II
(Microbial Techniques)
1. Rewrite the following sentences by choosing correct alternatives given below : 5
i) Hot air oven is used on ___________ principle.
a) Moist Heat b) Dry Heat
c) Radiation d) Desiccation
ii) HTST method of pasteurization is achieved at __________
a) 121°C for 20 min. b) 71.7°Cfor 15 sec.
c) 62.8°C for 30 mints. d) None of these
-3- SLR-M – 16
iii) ____________ method is used for staining of volutin granules.
a) Chance’s b) Manvel’s c) Albert’s d) Gram’s
iv) ______________ medium is selective as well as differential.
a) Potato Dextrose Agar b) Mac Conkey’s Agar
c) Saburaud’s Agar d) None of these
v) Alcohol acts as _____________ in Gram’s staining.
a) Mordant b) Primary Stain
c) Decoloriser d) Secondary Stain
2. Answer any five of the following : 10
i) Define pure culture and give methods for isolating pure culture.
ii) Define Diauxic growth.
iii) Give difference between moist heat and dry heat used for sterilization.
iv) Define synthetic media and give its two examples.
v) Give different methods used for cultivation of anaerobes.
vi) Define chemoorganotrophs give its example.
vii) Define Lyophilization.
3. A) Write short note on any two of the following : 10
i) Spread plate technique.
ii) Mechanism of cell wall staining of bacteria.
iii) Methods of pasteurization.
B) Answer any one of the following : 10
i) Explain growth and different phases of bacterial growth.
ii) Explain different methods used for maintenance of pure culture of
microorganisms.
_____________

Share This