Solapur University B.Sc Biochemical Techniques 2015 Question Paper

Solapur University
B.Sc. – II (Semester – IV) (Biotechnology) Examination, 2015
METHODS IN BIOTECHNOLOGY – II (Old)
Biochemical Techniques
Day and Date : Tuesday, 12-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
 N. B. : 1) All questions are compulsory.
2) Draw neat and labelled diagrams wherever necessary.
3) Figures to the right indicate full marks.
1. Write the following sentences by selecting most correct answer from given
alternatives. 10
i) The paper electrophoresis is a _____________ type of electrophoresis.
a) moving boundary b) zone
c) vertical d) all a), b) and c)
ii) The chemical nature of agarose used in electrophoresis is ____________
a) polysaccharide b) polypeptide
c) glycoprotein d) glycolipid
iii) In isoelectric focusing, proteins present in mixture will migrate to __________
and stop migration at that point.
a) cathode b) anode
c) its isoelectric point d) opposite end of sample loading
iv) In ascending paper chromatography the flow of mobile phase is always from
______________
a) top to bottom b) bottom to top
c) right to left d) left to right
v) _______________ chromatographic separation of macromolecules is based
only on molecular size.
a) Thin layer b) Ion-exchange
c) Affinity d) Gel permeation
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vi) ______________ is used for protein assay.
a) Biuret method
b) Antrone method
c) DNSA method
d) Phenol-H2SO4 method
vii) In native gel system used in proteomics all of the following are present except
_______________
a) Triton X-100 b) Acryl amide
c) Bis-acrylamide d) SDS
viii) ______________ of ions are measured by TOF analyzers of MALDI mass
spectrometer.
a) Mass b) Charge
c) Flight time d) Geometry
ix) The second electrophoresis in 2-D-gel electrophoresis is always ___________
a) isoelectric focusing
b) capillary electrophoresis
c) paper electrophoresis
d) SDS gel electrophoresis
x) The protein is concentrated by _______________ method/s.
a) freeze drying b) centrifugation
c) dialysis d) all a, b, and c
2. Answer any five of the following : 10
i) What is a stacking gel ? Why is it used in electrophoresis ?
ii) Distinguish between cation exchanger and anion exchanger.
iii) Give the role of SDS in electrophoresis.
iv) Define the terms – Proteome, Proteomics.
v) State the applications of western blotting technique.
vi) Explain the Ca2+ binding in blotting.
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3. A) Answer any two of the following : 6
i) What is the principle of affinity chromatography ? Give the properties of matrix
used in it.
ii) Describe the auto radiography of gels and blots.
iii) Illustrate Edman degradation method used in protein sequencing.
B) Write a note on working of starch gel electrophoresis. 4
4. Answer any two of the following : 10
i) Describe in detail thin layer chromatography.
ii) Explain the principles of different assay methods used in protein determination.
iii) Discuss in detail 2-D-gel electrophoresis.
5. Answer any two of the following : 10
i) With neat and labelled diagram describe Southern blotting technique.
ii) Illustrate the instrumentation of HPLC.
iii) Give a brief account of immunostaining method used in blotting.

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