Solapur University
B.Sc. – II (Semester – IV) (Biotechnology) Examination, 2015
METABOLISM – I (New)
Bioenergetics and Enzymology
Day and Date : Thursday, 14-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N. B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw labelled diagrams wherever necessary.
1. Write following sentences selecting most correct answer from given options : 10
1) Ribozymes are ______________ in nature that function like enzymes.
a) Proteins b) DNA c) RNA d) Polysaccharides
2) From thermodynamic point of view, biological cell is ___________ system.
a) Closed b) Open c) Adiabatic d) Isolated
3) For exothermic reactions enthalpy change of a system ( Δ H) is always
______________
a) > 0 b) = 0 c) < 0 d) constant
4) Condensation reaction between aldehyde and alcohol groups of two organic
compounds is called ______________
a) Clasein condensation b) Aldol condensation
c) Internal arrangement d) Group transfer reaction
5) Lipmann first demonstrated that ______________ functions in cyclic manner
to transfer energy from catabolic to anabolic reactions.
a) Phosphoenol pyruvate b) Phosphocreatine
c) Phosphoarginine d) Adenosine triphosphate
6) When an enzyme exists in more than one molecular forms in the same
organism, it is called as ______________
a) Coenzyme b) Holoenzyme
c) Apoenzyme d) Isoenzyme
7) Oxidising agents are the substances which ______________ in reaction.
a) are oxidised b) gain electrons
c) furnish electrons d) gain protons
P.T.O.
SLR-M – 45
8) Malonic acid reversibly inhibits the action of ______________ enzyme.
a) fumarase b) citrate synthase
c) succinate dehydrogenase d) malate dehydrogenase
9) Lactate dehydrogenase enzyme is composed of ______________ sub units.
a) 2 b) 4 c) 5 d) 6
10) Induced fit hypothesis of enzyme catalysis was proposed by _____________
a) Koshland b) Emil Fischer
c) Lineweaver and Burk d) Enzyme Commission
2. Answer any five from below : 10
1) State second law of thermodynamics. Give one example.
2) What is meant by standard free energy of a system ?
3) What are the drawbacks of Lock and Key mechanism for enzyme catalysis ?
4) What is absolute specificity of an enzyme ? Give one example.
5) What are coenzymes ? Give suitable examples.
6) Write and describe isomerisation reaction.
3. A) Answer any two : 6
1) Using Michaelis-Menten equation, derive Lineweaver-Burk plot equation.
2) Describe phosphoenol pyruvate as a high energy compound.
3) With suitable example write about biological half reaction.
B) How enzymes accelerate the reaction rate ? 4
4. Attempt any two : 10
1) How are enzymes classified ? Discuss each class in brief.
2) Derive Michaelis-Menten equation.
3) Discuss various modes of enzyme regulation.
5. Answer any two from below : 10
1) Write an account of high energy compounds of cell.
2) Discuss different types of enzyme inhibition.
3) Explain the role of various compounds involved in electron transport chain.
B.Sc. – II (Semester – IV) (Biotechnology) Examination, 2015
METABOLISM – I (New)
Bioenergetics and Enzymology
Day and Date : Thursday, 14-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N. B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw labelled diagrams wherever necessary.
1. Write following sentences selecting most correct answer from given options : 10
1) Ribozymes are ______________ in nature that function like enzymes.
a) Proteins b) DNA c) RNA d) Polysaccharides
2) From thermodynamic point of view, biological cell is ___________ system.
a) Closed b) Open c) Adiabatic d) Isolated
3) For exothermic reactions enthalpy change of a system ( Δ H) is always
______________
a) > 0 b) = 0 c) < 0 d) constant
4) Condensation reaction between aldehyde and alcohol groups of two organic
compounds is called ______________
a) Clasein condensation b) Aldol condensation
c) Internal arrangement d) Group transfer reaction
5) Lipmann first demonstrated that ______________ functions in cyclic manner
to transfer energy from catabolic to anabolic reactions.
a) Phosphoenol pyruvate b) Phosphocreatine
c) Phosphoarginine d) Adenosine triphosphate
6) When an enzyme exists in more than one molecular forms in the same
organism, it is called as ______________
a) Coenzyme b) Holoenzyme
c) Apoenzyme d) Isoenzyme
7) Oxidising agents are the substances which ______________ in reaction.
a) are oxidised b) gain electrons
c) furnish electrons d) gain protons
P.T.O.
SLR-M – 45
8) Malonic acid reversibly inhibits the action of ______________ enzyme.
a) fumarase b) citrate synthase
c) succinate dehydrogenase d) malate dehydrogenase
9) Lactate dehydrogenase enzyme is composed of ______________ sub units.
a) 2 b) 4 c) 5 d) 6
10) Induced fit hypothesis of enzyme catalysis was proposed by _____________
a) Koshland b) Emil Fischer
c) Lineweaver and Burk d) Enzyme Commission
2. Answer any five from below : 10
1) State second law of thermodynamics. Give one example.
2) What is meant by standard free energy of a system ?
3) What are the drawbacks of Lock and Key mechanism for enzyme catalysis ?
4) What is absolute specificity of an enzyme ? Give one example.
5) What are coenzymes ? Give suitable examples.
6) Write and describe isomerisation reaction.
3. A) Answer any two : 6
1) Using Michaelis-Menten equation, derive Lineweaver-Burk plot equation.
2) Describe phosphoenol pyruvate as a high energy compound.
3) With suitable example write about biological half reaction.
B) How enzymes accelerate the reaction rate ? 4
4. Attempt any two : 10
1) How are enzymes classified ? Discuss each class in brief.
2) Derive Michaelis-Menten equation.
3) Discuss various modes of enzyme regulation.
5. Answer any two from below : 10
1) Write an account of high energy compounds of cell.
2) Discuss different types of enzyme inhibition.
3) Explain the role of various compounds involved in electron transport chain.
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