Solapur University
B.Sc. – II (Semester – III) (Biotechnology) Examination, 2015
BIOTECHNIQUES (New)
Biophysical Techniques – I
Day and Date : Tuesday, 5-5-2015 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N. B. : 1) All questions are compulsory.
2) Draw neat and labelled diagram wherever necessary.
3) Figures to the right indicate full marks.
1. Rewrite the following sentences choosing the correct alternatives : 10
1) The amount of light absorbed by a material is proportional to the concentration
of the absorbing solution is referred as ______________
a) Beer’s law b) Lambert’s law
c) Poiseuille’s law d) Bouger’s law
2) Lead level in drinking water could be determined by using ______________
a) GC-MS b) AAS c) LC-MS d) GLC
3) _________ is the process of enlarging something only in appearance not in
physical size.
a) Resolving power b) Numerical aperture
c) Magnification d) Focal length
4) ______________ is present at the upper end of the body tube.
a) Mechanical stage b) Nose Piece
c) Fine adjustment Knob d) Eyepiece
5) A biologically insignificant radioactive isotope is ______________
a) 15O b) 35S c) 15N d) 32P
6) ______________ is the method used for measurement of radioactivity based
on excitation of gas.
a) GM counters b) Scintillation counters
c) Proportional counters d) Ionization chamber
7) Ratio of ______________ determines the stability of isotope in nature.
a) Electron to Proton b) Electron to Neutron
c) Neutron to Proton d) Meson to Neutron
P.T.O.
SLR-M – 37
8) ______________ is the technique responsible for understanding of cellular
make up.
a) Radioactivity b) Electrophoresis
c) Chromatography d) Ultra-centrifugation
9) Individual cells can be measured by the technique ______________
a) Flow cytometry b) Dosimetry
c) Colorimetry d) Autoradiography
10) X-ray diffraction fails to detect the presence of substances ______________
a) Containing higher concentration of carbon
b) Comprising less than 5% of a mixture
c) Containing a magnetic field
d) Comprising elements with two or more isotopes
2. Answer the following (any five) : 10
1) Write a short note on electromagnetic spectrum.
2) Define acids and bases.
3) Comment on convex lens.
4) Absorbed dose (D).
5) Enlist the applications of circular Dichroism.
6) Properties of alpha(α ) particles.
3. A) Answer the following (any two) : 6
1) Describe pH indicators for pH measurement.
2) Explain chromatic and achromatic aberrations.
3) Write a note on swinging bucket rotors.
B) Discuss ionization chamber for measurement of radioactivity. 4
4. Answer the following (any two) : 10
1) Describe the construction of colorimeter.
2) Discuss construction and working of X-ray difractometer.
3) Explain image formation in compound light microscope.
5. Answer the following (any two) : 10
1) Explain in detail atomic absorption spectroscopy.
2) Describe analytical ultracentrifugation.
3) Discuss GM counters for measurement of radioactivity and enlist the biological applications of radioisotopes.
B.Sc. – II (Semester – III) (Biotechnology) Examination, 2015
BIOTECHNIQUES (New)
Biophysical Techniques – I
Day and Date : Tuesday, 5-5-2015 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N. B. : 1) All questions are compulsory.
2) Draw neat and labelled diagram wherever necessary.
3) Figures to the right indicate full marks.
1. Rewrite the following sentences choosing the correct alternatives : 10
1) The amount of light absorbed by a material is proportional to the concentration
of the absorbing solution is referred as ______________
a) Beer’s law b) Lambert’s law
c) Poiseuille’s law d) Bouger’s law
2) Lead level in drinking water could be determined by using ______________
a) GC-MS b) AAS c) LC-MS d) GLC
3) _________ is the process of enlarging something only in appearance not in
physical size.
a) Resolving power b) Numerical aperture
c) Magnification d) Focal length
4) ______________ is present at the upper end of the body tube.
a) Mechanical stage b) Nose Piece
c) Fine adjustment Knob d) Eyepiece
5) A biologically insignificant radioactive isotope is ______________
a) 15O b) 35S c) 15N d) 32P
6) ______________ is the method used for measurement of radioactivity based
on excitation of gas.
a) GM counters b) Scintillation counters
c) Proportional counters d) Ionization chamber
7) Ratio of ______________ determines the stability of isotope in nature.
a) Electron to Proton b) Electron to Neutron
c) Neutron to Proton d) Meson to Neutron
P.T.O.
SLR-M – 37
8) ______________ is the technique responsible for understanding of cellular
make up.
a) Radioactivity b) Electrophoresis
c) Chromatography d) Ultra-centrifugation
9) Individual cells can be measured by the technique ______________
a) Flow cytometry b) Dosimetry
c) Colorimetry d) Autoradiography
10) X-ray diffraction fails to detect the presence of substances ______________
a) Containing higher concentration of carbon
b) Comprising less than 5% of a mixture
c) Containing a magnetic field
d) Comprising elements with two or more isotopes
2. Answer the following (any five) : 10
1) Write a short note on electromagnetic spectrum.
2) Define acids and bases.
3) Comment on convex lens.
4) Absorbed dose (D).
5) Enlist the applications of circular Dichroism.
6) Properties of alpha(α ) particles.
3. A) Answer the following (any two) : 6
1) Describe pH indicators for pH measurement.
2) Explain chromatic and achromatic aberrations.
3) Write a note on swinging bucket rotors.
B) Discuss ionization chamber for measurement of radioactivity. 4
4. Answer the following (any two) : 10
1) Describe the construction of colorimeter.
2) Discuss construction and working of X-ray difractometer.
3) Explain image formation in compound light microscope.
5. Answer the following (any two) : 10
1) Explain in detail atomic absorption spectroscopy.
2) Describe analytical ultracentrifugation.
3) Discuss GM counters for measurement of radioactivity and enlist the biological applications of radioisotopes.
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