Solapur University
B.Sc. – II (Biotechnology) (Semester – IV) Examination, 2015
METABOLISM – II (New)
Day and Date : Friday, 15-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N. B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Write biochemical reactions wherever necessary.
1. Write the following sentences by choosing the most correct alternative from
given options : 10
1) Succinyl – CoA is cleaved by ___________ enzyme in TCA cycle.
a) Succinate dehydrogenase
b) Succinate thiokinase
c) Succinate lyase
d) Succinate thioesterase
2) The reaction of conversion of isocitrate to α -ketoglutarate in TCA cycle is
______________
a) Oxidative decarboxylation
b) Condensation
c) Dehydrogenation
d) Substrate level phosphorylation
3) The conversion of __________ in glycolysis is an irreversible step.
a) Glucose 6 phosphate to fructose 6-phosphate
b) Glyceraldehyde 3-phosphate to dihydroxyacetone phosphate
c) 3-phosphoglycerate to 2-phosphoglycerate
d) Fructose 6-phosphate to fructose 1, 6-bisphosphate
SLR-M – 46 -2-
4) ___________ is the first stable product formed in C3 pathway of CO2 fixation.
a) Phosphoglyceric acid b) Phosphoglyceraldehyde
c) Oxaloacetate d) Ribulose bisphosphate
5) The photorespiration yields __________
a) Phosphoglycerate b) Phosphoglycolate
c) Both a) and b) d) None of these
6) ____________ contributes nitrogen atoms in the biosynthesis of purine and
pyrimidine rings.
a) Aspartate b) Carbamoyl phosphate
c) Carbon dioxide d) Glutamine
7) ____________ is the end-product of purine catabolism in human.
a) CO2 b) Lactic acid
c) Ethanol d) Uric acid
8) ____________ molecules of ATP can be derived from each acetyl CoA
molecule entering the Kreb’s cycle.
a) 6 b) 12 c) 18 d) 38
9) Increased concentration of ____________ inhibits the beta oxidation of fatty
acids.
a) ATP b) OAA
c) Malonyl CoA d) Citrate
10) ___________ is the prosthetic group of NADH-COQ reductase.
a) Heme b) NADP c) FMN d) Mg++
2. Answer any five of the following : 10
1) Define glycolysis and give its energetics.
2) Write about the inhibitors of electron transport chain.
3) Define photosystem and differentiate between PS-I and PS-II.
4) Give an outline of amino acid biosynthesis.
5) Write about oxidation of fatty acids containing odd numbered carbon chains.
6) How are the fatty acids transported into mitochondria ?
-3- SLR-M – 46
3. A) Answer any two of the following : 6
1) Describe the ATP synthetase complex.
2) Write a note on glycogen synthesis.
3) Describe the regulation of cholesterol metabolism.
B) Discuss the fates of pyruvate during metabolism. 4
4. Answer any two of the following : 10
1) Write an account on pentose phosphate pathway.
2) Discuss the biosynthesis of purine nucleotides.
3) Describe the TCA cycle and explain how it is regulated.
5. Answer any two of the following : 10
1) Explain the mechanism of CO2 fixation in C4 plants.
2) Write an account on beta oxidation of fatty acids.
3) Discuss in detail the light reaction of photosynthesis.
B.Sc. – II (Biotechnology) (Semester – IV) Examination, 2015
METABOLISM – II (New)
Day and Date : Friday, 15-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N. B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Write biochemical reactions wherever necessary.
1. Write the following sentences by choosing the most correct alternative from
given options : 10
1) Succinyl – CoA is cleaved by ___________ enzyme in TCA cycle.
a) Succinate dehydrogenase
b) Succinate thiokinase
c) Succinate lyase
d) Succinate thioesterase
2) The reaction of conversion of isocitrate to α -ketoglutarate in TCA cycle is
______________
a) Oxidative decarboxylation
b) Condensation
c) Dehydrogenation
d) Substrate level phosphorylation
3) The conversion of __________ in glycolysis is an irreversible step.
a) Glucose 6 phosphate to fructose 6-phosphate
b) Glyceraldehyde 3-phosphate to dihydroxyacetone phosphate
c) 3-phosphoglycerate to 2-phosphoglycerate
d) Fructose 6-phosphate to fructose 1, 6-bisphosphate
SLR-M – 46 -2-
4) ___________ is the first stable product formed in C3 pathway of CO2 fixation.
a) Phosphoglyceric acid b) Phosphoglyceraldehyde
c) Oxaloacetate d) Ribulose bisphosphate
5) The photorespiration yields __________
a) Phosphoglycerate b) Phosphoglycolate
c) Both a) and b) d) None of these
6) ____________ contributes nitrogen atoms in the biosynthesis of purine and
pyrimidine rings.
a) Aspartate b) Carbamoyl phosphate
c) Carbon dioxide d) Glutamine
7) ____________ is the end-product of purine catabolism in human.
a) CO2 b) Lactic acid
c) Ethanol d) Uric acid
8) ____________ molecules of ATP can be derived from each acetyl CoA
molecule entering the Kreb’s cycle.
a) 6 b) 12 c) 18 d) 38
9) Increased concentration of ____________ inhibits the beta oxidation of fatty
acids.
a) ATP b) OAA
c) Malonyl CoA d) Citrate
10) ___________ is the prosthetic group of NADH-COQ reductase.
a) Heme b) NADP c) FMN d) Mg++
2. Answer any five of the following : 10
1) Define glycolysis and give its energetics.
2) Write about the inhibitors of electron transport chain.
3) Define photosystem and differentiate between PS-I and PS-II.
4) Give an outline of amino acid biosynthesis.
5) Write about oxidation of fatty acids containing odd numbered carbon chains.
6) How are the fatty acids transported into mitochondria ?
-3- SLR-M – 46
3. A) Answer any two of the following : 6
1) Describe the ATP synthetase complex.
2) Write a note on glycogen synthesis.
3) Describe the regulation of cholesterol metabolism.
B) Discuss the fates of pyruvate during metabolism. 4
4. Answer any two of the following : 10
1) Write an account on pentose phosphate pathway.
2) Discuss the biosynthesis of purine nucleotides.
3) Describe the TCA cycle and explain how it is regulated.
5. Answer any two of the following : 10
1) Explain the mechanism of CO2 fixation in C4 plants.
2) Write an account on beta oxidation of fatty acids.
3) Discuss in detail the light reaction of photosynthesis.
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