Solapur University
B.Sc. (Part – II) (Semester – IV) Examination, 2015
BIOTECHNOLOGY
Molecular Biology – II (Gene Regulation and Expression) (Old)
Day and Date : Monday, 11-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to the right side indicates full marks.
1. Rewrite the sentence using correct alternative given below : 10
1) In eukaryotic transcription process, ______________ is responsible for mRNA
synthesis.
a) RNA Pol I b) RNA Pol II c) RNA Pol III d) Poly A RNA Pol
2) In prokaryotic transcription process ______________ is responsible for binding
a promoter region.
a) σ factor b) rho factor c) TF – II D d) EL II D
3) In lac operon, regulatory protein binds to ______________ sequences.
a) Promoter b) Operator c) Terminator d) Attenuator
4) ______________ is inhibitor of transcription process.
a) Streptomycin b) Actinomycin c) Ampicillin d) Tetracycline
5) ______________ is transcribed but not translated.
a) tRNA b) mRNA c) rRNA d) Both a) and c)
6) ______________ is not a termination codon.
a) AUG b) UAG c) UGA d) UAA
7) In lac operon Lac y gene encodes______________
a) Galactosidase b) Permease c) Transacetylase d) Lactamase
8) Shine Dalgarno sequences are known as ______________
a) Initiator sequences b) Ribosome binding site
c) Promoter sequences d) Operator sequences
9) Attachment of carbohydrates to proteins is known as ______________
a) Glycosylation b) Glycolysis c) Glycogenosis d) Glyconeogenesis
10) A cistron refers to ______________
a) Coding region b) Non-coding region
c) Mutated region d) Replicative region
P.T.O.
SLR-M – 48
2. Answer any five of the following : 10
i) Sn RNPs
ii) trpE
iii) 16S rRNA
iv) Streptomycin
v) Heat shock proteins
vi) Regulator protein.
3. A) Answer any two of the following : 6
i) Write a note on mRNA splicing.
ii) Add a note on termination of transcription in prokaryotes.
iii) Structure of tRNA molecule.
B) Describe structure and function of ribosomes. 4
4. Answer any two of the following : 10
i) Describe the mechanism of transcription in eukaryotes.
ii) Explain the mechanism of translation in prokaryotes.
iii) Explain the regulation of Lac operon.
5. Answer any two of the following : 10
i) Write a note on post translation modification in eukaryotes.
ii) Add a note on factors involved in prokaryotic transcription.
iii) Explain Trp operon in detail.
B.Sc. (Part – II) (Semester – IV) Examination, 2015
BIOTECHNOLOGY
Molecular Biology – II (Gene Regulation and Expression) (Old)
Day and Date : Monday, 11-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to the right side indicates full marks.
1. Rewrite the sentence using correct alternative given below : 10
1) In eukaryotic transcription process, ______________ is responsible for mRNA
synthesis.
a) RNA Pol I b) RNA Pol II c) RNA Pol III d) Poly A RNA Pol
2) In prokaryotic transcription process ______________ is responsible for binding
a promoter region.
a) σ factor b) rho factor c) TF – II D d) EL II D
3) In lac operon, regulatory protein binds to ______________ sequences.
a) Promoter b) Operator c) Terminator d) Attenuator
4) ______________ is inhibitor of transcription process.
a) Streptomycin b) Actinomycin c) Ampicillin d) Tetracycline
5) ______________ is transcribed but not translated.
a) tRNA b) mRNA c) rRNA d) Both a) and c)
6) ______________ is not a termination codon.
a) AUG b) UAG c) UGA d) UAA
7) In lac operon Lac y gene encodes______________
a) Galactosidase b) Permease c) Transacetylase d) Lactamase
8) Shine Dalgarno sequences are known as ______________
a) Initiator sequences b) Ribosome binding site
c) Promoter sequences d) Operator sequences
9) Attachment of carbohydrates to proteins is known as ______________
a) Glycosylation b) Glycolysis c) Glycogenosis d) Glyconeogenesis
10) A cistron refers to ______________
a) Coding region b) Non-coding region
c) Mutated region d) Replicative region
P.T.O.
SLR-M – 48
2. Answer any five of the following : 10
i) Sn RNPs
ii) trpE
iii) 16S rRNA
iv) Streptomycin
v) Heat shock proteins
vi) Regulator protein.
3. A) Answer any two of the following : 6
i) Write a note on mRNA splicing.
ii) Add a note on termination of transcription in prokaryotes.
iii) Structure of tRNA molecule.
B) Describe structure and function of ribosomes. 4
4. Answer any two of the following : 10
i) Describe the mechanism of transcription in eukaryotes.
ii) Explain the mechanism of translation in prokaryotes.
iii) Explain the regulation of Lac operon.
5. Answer any two of the following : 10
i) Write a note on post translation modification in eukaryotes.
ii) Add a note on factors involved in prokaryotic transcription.
iii) Explain Trp operon in detail.
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