Solapur University
B.Sc. – II (Semester – IV) Examination, 2015
BIOTECHNOLOGY (New)
Molecular Biology
Gene Regulation
Day and Date : Monday, 11-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N.B. : 1) All the questions are compulsory.
2) Figures to the right indicates full marks.
3) Draw diagram wherever necessary.
1. Rewrite the sentences using correct alternatives : 10
1) Nuclear DNA sends information for protein synthesis with the help of _____________
a) tRNA b) mRNA c) rRNA d) all of these
2) Lac operon of E. coli is of _____________ type.
a) Monocistronic b) Inducible c) Repressible d) Both a) and c)
3) Transcription involves synthesis of _____________
a) tRNA b) mRNA c) rRNA d) all of these
4) Enzymes required for transcription is _____________
a) RNA-ase b) Transnuclease
c) RNA polymerase d) DNA-polymerase
5) t-RNA attaches amino acid at its _____________
a) 3′ end b) 5′ end c) Anticodon d) Loop
6) Inducible and Represser operon are respectively _____________
a) Lac operon and Tryptophan operon
b) Lac operon in both
c) Tryptophan operon both type
d) None of these
7) During elongation of polypeptide chain sigma factor is _____________
a) Functionless b) Released for re-use
c) Retained for specific function d) Required during closing of chain
8) RNA polymerase I transcribes _____________
a) m-RNA b) r-RNA c) t-RNA d) sn-RNA
9) Those stretches of DNA that do code for amino acids in the protein are called _____________
a) Introns b) Poly A tail c) Exons d) Split gene
10) The removal introns from exons is done with the _____________
a) Spliceosome b) RNA polymerase
c) DNA polymerase d) Proteinases
2. Answer the following (any 5) : 10
1) What are split genes ?
2) Write a note on ribosome structure.
3) What is Fidelity of translation ?
4) What are transcriptional repressors ?
5) Describe regulation of Trp operon.
6) What are introns and exons ?
3. A) Answer the following (any 2) : 6
1) Describe the process of initiation of transcription in prokaryotes.
2) Write a note on eukaryotic RNA polymerases.
3) Describe various inhibitors of protein synthesis.
B) Describe the steps involved in protein synthesis in eukaryotes. 4
4. Answer the following (any 2) : 10
1) Describe Lac Operon in detail.
2) Describe the process of transcription in eukaryotes.
3) Describe translation-dependent regulation of m-RNA and add a note on protein stability.
5. Answer the following (any 2) : 10
1) What is conserved mechanism of regulation ?
2) Describe the RNA processing in eukaryotes.
3) Explain with an example the signal transduction in transcriptional regulation.
B.Sc. – II (Semester – IV) Examination, 2015
BIOTECHNOLOGY (New)
Molecular Biology
Gene Regulation
Day and Date : Monday, 11-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N.B. : 1) All the questions are compulsory.
2) Figures to the right indicates full marks.
3) Draw diagram wherever necessary.
1. Rewrite the sentences using correct alternatives : 10
1) Nuclear DNA sends information for protein synthesis with the help of _____________
a) tRNA b) mRNA c) rRNA d) all of these
2) Lac operon of E. coli is of _____________ type.
a) Monocistronic b) Inducible c) Repressible d) Both a) and c)
3) Transcription involves synthesis of _____________
a) tRNA b) mRNA c) rRNA d) all of these
4) Enzymes required for transcription is _____________
a) RNA-ase b) Transnuclease
c) RNA polymerase d) DNA-polymerase
5) t-RNA attaches amino acid at its _____________
a) 3′ end b) 5′ end c) Anticodon d) Loop
6) Inducible and Represser operon are respectively _____________
a) Lac operon and Tryptophan operon
b) Lac operon in both
c) Tryptophan operon both type
d) None of these
7) During elongation of polypeptide chain sigma factor is _____________
a) Functionless b) Released for re-use
c) Retained for specific function d) Required during closing of chain
8) RNA polymerase I transcribes _____________
a) m-RNA b) r-RNA c) t-RNA d) sn-RNA
9) Those stretches of DNA that do code for amino acids in the protein are called _____________
a) Introns b) Poly A tail c) Exons d) Split gene
10) The removal introns from exons is done with the _____________
a) Spliceosome b) RNA polymerase
c) DNA polymerase d) Proteinases
2. Answer the following (any 5) : 10
1) What are split genes ?
2) Write a note on ribosome structure.
3) What is Fidelity of translation ?
4) What are transcriptional repressors ?
5) Describe regulation of Trp operon.
6) What are introns and exons ?
3. A) Answer the following (any 2) : 6
1) Describe the process of initiation of transcription in prokaryotes.
2) Write a note on eukaryotic RNA polymerases.
3) Describe various inhibitors of protein synthesis.
B) Describe the steps involved in protein synthesis in eukaryotes. 4
4. Answer the following (any 2) : 10
1) Describe Lac Operon in detail.
2) Describe the process of transcription in eukaryotes.
3) Describe translation-dependent regulation of m-RNA and add a note on protein stability.
5. Answer the following (any 2) : 10
1) What is conserved mechanism of regulation ?
2) Describe the RNA processing in eukaryotes.
3) Explain with an example the signal transduction in transcriptional regulation.
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