Solapur University
B.Sc. – II (Biotechnology) (Semester – IV) Examination, 2015
TISSUE TECHNIQUES
Animal Tissue Culture (New)
Day and Date : Wednesday, 13-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to right indicate full marks.
3) Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 10
1) Culture of _________ is more difficult than micro-organisms.
a) Animal cell b) Plant cell c) Yeast cell d) Fungus cell
2) Hella cell line is derived from ___________ cell line.
a) Stomach b) Cervical c) Lung d) Blood
3) Which of the following behavior not shown by normal cell in culture ?
a) Contact inhibition b) Monolayer formation
c) Uncontrolled cell division d) Encourage dependent
4) Along with nutritional and hormonal factors ___________ factor is also essential
for survival of cell in culture.
a) Insulin b) Lipids c) Proteins d) Stromal
5) Plasma clot technique is also known as __________ technique.
a) Watch glass b) Grid c) Raft d) Cyclic exposure
6) In primary culture, cell divide to give same type of cells by __________
process.
a) Differentiation b) Proliferation
c) Cultivation d) Initiation
7) When all the cells in culture are in same phase of growth; the process is
known as __________
a) Trypsinization b) Primary cell culture
c) Cell synchronization d) Apoptosis
SLR-M – 44
8) Colony formation at low cell density or ___________ is the preferred method
for analyzing cell proliferation and survival.
a) Trypsinization b) Apoptosis
c) Cell migration d) Plating efficiency
9) __________ portion of skin is used to produce artificial skin.
a) Epidermis b) Dermis c) Epithelial d) Endothelial
10) In primary cell culture cell divide to give different type of cell by _________
process.
a) Cultivation b) Differentiation
c) Initiation d) Proliferation
2. Answer the following (any five) : 10
1) Write a note on history of ATC.
2) Describe in brief laminar air flow.
3) Define primary cell culture.
4) Write a note on plating efficiency of cultured cells.
5) Explain in brief monoclonal antibodies.
6) Write a note on washing of glasswares.
3. A) Answer the following (any two) : 6
1) Write a note on morphology of cultured cells.
2) Write a note on cultured based vaccine.
3) Explain in brief BSS.
B) Explain physiological media components. 4
4. Answer the following (any two) : 10
1) Describe the culture of stem cells.
2) Explain equipments used in animal cell culture.
3) Give details of cell synchronization.
5. Answer the following (any two) : 10
1) Discuss in detail synthetic media.
2) Describe in detail measurement of senescence.
3) Give details of applications of animal cell culture.
B.Sc. – II (Biotechnology) (Semester – IV) Examination, 2015
TISSUE TECHNIQUES
Animal Tissue Culture (New)
Day and Date : Wednesday, 13-5-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to right indicate full marks.
3) Draw neat and labeled diagrams.
1. Rewrite the following sentences by choosing correct alternatives. 10
1) Culture of _________ is more difficult than micro-organisms.
a) Animal cell b) Plant cell c) Yeast cell d) Fungus cell
2) Hella cell line is derived from ___________ cell line.
a) Stomach b) Cervical c) Lung d) Blood
3) Which of the following behavior not shown by normal cell in culture ?
a) Contact inhibition b) Monolayer formation
c) Uncontrolled cell division d) Encourage dependent
4) Along with nutritional and hormonal factors ___________ factor is also essential
for survival of cell in culture.
a) Insulin b) Lipids c) Proteins d) Stromal
5) Plasma clot technique is also known as __________ technique.
a) Watch glass b) Grid c) Raft d) Cyclic exposure
6) In primary culture, cell divide to give same type of cells by __________
process.
a) Differentiation b) Proliferation
c) Cultivation d) Initiation
7) When all the cells in culture are in same phase of growth; the process is
known as __________
a) Trypsinization b) Primary cell culture
c) Cell synchronization d) Apoptosis
SLR-M – 44
8) Colony formation at low cell density or ___________ is the preferred method
for analyzing cell proliferation and survival.
a) Trypsinization b) Apoptosis
c) Cell migration d) Plating efficiency
9) __________ portion of skin is used to produce artificial skin.
a) Epidermis b) Dermis c) Epithelial d) Endothelial
10) In primary cell culture cell divide to give different type of cell by _________
process.
a) Cultivation b) Differentiation
c) Initiation d) Proliferation
2. Answer the following (any five) : 10
1) Write a note on history of ATC.
2) Describe in brief laminar air flow.
3) Define primary cell culture.
4) Write a note on plating efficiency of cultured cells.
5) Explain in brief monoclonal antibodies.
6) Write a note on washing of glasswares.
3. A) Answer the following (any two) : 6
1) Write a note on morphology of cultured cells.
2) Write a note on cultured based vaccine.
3) Explain in brief BSS.
B) Explain physiological media components. 4
4. Answer the following (any two) : 10
1) Describe the culture of stem cells.
2) Explain equipments used in animal cell culture.
3) Give details of cell synchronization.
5. Answer the following (any two) : 10
1) Discuss in detail synthetic media.
2) Describe in detail measurement of senescence.
3) Give details of applications of animal cell culture.
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