Solapur University
B.Sc. – I (Semester – II) (Biotechnology) (Old) Examination, 2015
ENVIRONMENTAL POLLUTION AND MICROBIAL TECHNIQUES
Microbial Techniques (Paper – II)
Day and Date : Wednesday, 22-4-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N. B. : 1) All the questions are compulsory.
2) Figures to right indicate full marks.
1. Rewrite the sentences using correct alternatives : 10
i) Alcohol acts as a ________________ in Gram’s staining.
a) Mordant b) Primary stain
c) Decolouriser d) Secondary stain
ii) U.V. rays are effective against bacteria at _______________
a) 150 nm b) 390 nm c) 102 nm d) 265 nm
iii) _______________ medium is selective as well as differential.
a) Potato Dextrose Agar b) Mac Conkey’s
c) Sabouraud’s d) None of these
iv) Blood agar media is example of _______________
a) Natural b) Artificial c) Both d) None of these
v) _____________ method is known for acid fast staining.
a) Manvel’s b) Albert’s c) Gram’s d) Ziehl-Neelsen
vi) Growth in the cell population in which all cells divide the same time known as
__________ growth.
a) Diauxic b) Simple c) Synchronous d) Periodic
vii) Pasteurisation is ______________
a) Sterilization b) Partial sterilization
c) Sanitisation d) Disinfection
viii) _________ is acts as mordant in capsule staining.
a) Alcohol b) Phenol c) FeCl3 d) FeCl2
SLR-M – 22
ix) Rosenthal method is used for culturing ________________
a) Aerobic bacteria b) Anaerobic bacteria
c) Both of these d) None of these
x) The organism which use CO2 as carbon source and light as energy source
belongs to ____________
a) Phototroph b) Chemoautotroph
c) Photoheterotroph d) Chemoheterotroph
2. Write short answers (any five) : 10
1) Give examples of disinfectants.
2) Define pasteurisation.
3) Define photolithotrophs.
4) Give two examples of selective media.
5) Define pure culture.
6) Define basic stain and give its examples.
3. A) Answer any two of the following : 6
1) Explain diauxic growth with neat labelled diagram.
2) Write short note on Autoclave.
3) Explain different methods used for cultivation of anaerobes.
B) What is meant by pure culture ? Discuss in detail the methods of isolation of pure culture. 4
4. Write brief note on (any two) : 10
1) Physical agents used for sterilization.
2) Growth phases in bacterial culture.
3) What is meant by culture media ? Write in detail about differential and selective
media with example.
5. Write a brief note on (any two) : 10
1) Different theories to explain Gram staining mechanism.
2) Write on account on sterilization of filtration.
3) Laboratory methods of determination of growth.
B.Sc. – I (Semester – II) (Biotechnology) (Old) Examination, 2015
ENVIRONMENTAL POLLUTION AND MICROBIAL TECHNIQUES
Microbial Techniques (Paper – II)
Day and Date : Wednesday, 22-4-2015 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
N. B. : 1) All the questions are compulsory.
2) Figures to right indicate full marks.
1. Rewrite the sentences using correct alternatives : 10
i) Alcohol acts as a ________________ in Gram’s staining.
a) Mordant b) Primary stain
c) Decolouriser d) Secondary stain
ii) U.V. rays are effective against bacteria at _______________
a) 150 nm b) 390 nm c) 102 nm d) 265 nm
iii) _______________ medium is selective as well as differential.
a) Potato Dextrose Agar b) Mac Conkey’s
c) Sabouraud’s d) None of these
iv) Blood agar media is example of _______________
a) Natural b) Artificial c) Both d) None of these
v) _____________ method is known for acid fast staining.
a) Manvel’s b) Albert’s c) Gram’s d) Ziehl-Neelsen
vi) Growth in the cell population in which all cells divide the same time known as
__________ growth.
a) Diauxic b) Simple c) Synchronous d) Periodic
vii) Pasteurisation is ______________
a) Sterilization b) Partial sterilization
c) Sanitisation d) Disinfection
viii) _________ is acts as mordant in capsule staining.
a) Alcohol b) Phenol c) FeCl3 d) FeCl2
SLR-M – 22
ix) Rosenthal method is used for culturing ________________
a) Aerobic bacteria b) Anaerobic bacteria
c) Both of these d) None of these
x) The organism which use CO2 as carbon source and light as energy source
belongs to ____________
a) Phototroph b) Chemoautotroph
c) Photoheterotroph d) Chemoheterotroph
2. Write short answers (any five) : 10
1) Give examples of disinfectants.
2) Define pasteurisation.
3) Define photolithotrophs.
4) Give two examples of selective media.
5) Define pure culture.
6) Define basic stain and give its examples.
3. A) Answer any two of the following : 6
1) Explain diauxic growth with neat labelled diagram.
2) Write short note on Autoclave.
3) Explain different methods used for cultivation of anaerobes.
B) What is meant by pure culture ? Discuss in detail the methods of isolation of pure culture. 4
4. Write brief note on (any two) : 10
1) Physical agents used for sterilization.
2) Growth phases in bacterial culture.
3) What is meant by culture media ? Write in detail about differential and selective
media with example.
5. Write a brief note on (any two) : 10
1) Different theories to explain Gram staining mechanism.
2) Write on account on sterilization of filtration.
3) Laboratory methods of determination of growth.
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