Solapur University
B.Sc. – III (Semester – V) Examination, 2015
BIOTECHNOLOGY
Fermentation Technology
Day and Date : Tuesday, 7-4-2015 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N. B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Rewrite the sentences using correct alternative given below : 10
1) Hydrolysis of starch is required in ethanol fermentation as saccharomyses
cerevisiae has ______________
a) no α -amylases b) no β-amylases
c) no glucoamylases d) all of these
2) In ______________ fermentation cobalt and 5, 6-dimethylbenimidazole must
be added as supplement.
a) citric acid b) vitamin B12 c) ethanol d) amylase
3) Fermentation economics is based on ______________
a) medium constituent b) incubation period
c) recovery d) all of these
4) In alcohol industry liquefaction of starch before the addition of malt
saccharification carried by ______________
a) α-amylases b) protease c) lipase d) nuclease
5) Soy meal used in fermentation has ______________
a) 50% proteins and 30% carbohydrates
b) 30% proteins and 50% carbohydrates
c) very less proteins
d) very high carbohydrates
6) Through the reverse osmosis ___________ sized particles are separated.
a) 0.0001 – 0.001 μm b) 0.001 – 0.1 μm
c) 0.1 – 10μm d) 10 – 100 μm
7) Above ______________ citric acid crystallizes as anhydrous acid.
a) 34°C b) 36.5°C c) 40°C d) 27°C
8) Head space at the top of fermentor allows liquid medium for ____________
a) splashing b) foaming c) aeration d) all of these
P.T.O.
SLR-M – 55
9) The sterilization of air in fermentation industry is widely carried out by
__________
a) Heat b) U.V.
c) Filtration d) Electrostatic precipitation
10) Primary screening of organic acid producer carried by ______________ in
nutrient agar.
a) addition of pH indicator b) addition of calcium carbonate
c) both of these d) none of these
2. Explain any five of given below : 10
1) Agitation
2) Transduction
3) Precipitation
4) Screening
5) Scale up
6) Vitamin.
3. A) Write the short answer of any two : 6
1) Media sterilization.
2) Microbial assay.
3) Applications of computer in fermentation.
B) Write an essay on centrifugation for product recovery. 4
4. Answer any two of the following : 10
1) Write an essay on preservation and maintenance of industrial microbes.
2) Write an essay on penicillin fermentation.
3) Write an essay on components of typical fermentor.
5. Answer any two of the following : 10
1) Write an essay on primary screening.
2) Write an essay on vitamin B12 fermentation.
3) Write an essay on amylase fermentation.
B.Sc. – III (Semester – V) Examination, 2015
BIOTECHNOLOGY
Fermentation Technology
Day and Date : Tuesday, 7-4-2015 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N. B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Rewrite the sentences using correct alternative given below : 10
1) Hydrolysis of starch is required in ethanol fermentation as saccharomyses
cerevisiae has ______________
a) no α -amylases b) no β-amylases
c) no glucoamylases d) all of these
2) In ______________ fermentation cobalt and 5, 6-dimethylbenimidazole must
be added as supplement.
a) citric acid b) vitamin B12 c) ethanol d) amylase
3) Fermentation economics is based on ______________
a) medium constituent b) incubation period
c) recovery d) all of these
4) In alcohol industry liquefaction of starch before the addition of malt
saccharification carried by ______________
a) α-amylases b) protease c) lipase d) nuclease
5) Soy meal used in fermentation has ______________
a) 50% proteins and 30% carbohydrates
b) 30% proteins and 50% carbohydrates
c) very less proteins
d) very high carbohydrates
6) Through the reverse osmosis ___________ sized particles are separated.
a) 0.0001 – 0.001 μm b) 0.001 – 0.1 μm
c) 0.1 – 10μm d) 10 – 100 μm
7) Above ______________ citric acid crystallizes as anhydrous acid.
a) 34°C b) 36.5°C c) 40°C d) 27°C
8) Head space at the top of fermentor allows liquid medium for ____________
a) splashing b) foaming c) aeration d) all of these
P.T.O.
SLR-M – 55
9) The sterilization of air in fermentation industry is widely carried out by
__________
a) Heat b) U.V.
c) Filtration d) Electrostatic precipitation
10) Primary screening of organic acid producer carried by ______________ in
nutrient agar.
a) addition of pH indicator b) addition of calcium carbonate
c) both of these d) none of these
2. Explain any five of given below : 10
1) Agitation
2) Transduction
3) Precipitation
4) Screening
5) Scale up
6) Vitamin.
3. A) Write the short answer of any two : 6
1) Media sterilization.
2) Microbial assay.
3) Applications of computer in fermentation.
B) Write an essay on centrifugation for product recovery. 4
4. Answer any two of the following : 10
1) Write an essay on preservation and maintenance of industrial microbes.
2) Write an essay on penicillin fermentation.
3) Write an essay on components of typical fermentor.
5. Answer any two of the following : 10
1) Write an essay on primary screening.
2) Write an essay on vitamin B12 fermentation.
3) Write an essay on amylase fermentation.
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