Solapur University Question Paper
M.Sc. (Part – II) (Semester – IV) Examination, 2014
BIOINFORMATICS
Advanced Molecular Biology (Paper – III)
Day and Date : Saturday, 26-4-2014 Total Marks : 100
Time : 3.00 p.m. to 6.00 p.m.
Instructions :1) Part – I, question 1 is compulsory.
2) Attempt any four questions from Part – II.
3) Figures to the right indicate full marks.
4) Answers to the Part – I and Part – II are to be written in
same answer book only.
PART – I
1. A) Choose the correct answer from given alternatives : 10
I) The thermostable form of DNA polymerase used in PCR cycles is
_____________
a) Paq b) Baq c) Taq d) Maq
II) __________ blotting technique involves transfer of RNA molecules onto
membranes for the detection of specific sequences by hybridization.
a) Northern blotting b) Southern blotting
c) Western blotting d) South Western blotting
III) Relative expression levels of thousands of genes can be visualized
using _____________ technology.
a) Autoradiography b) DNA fingerprinting
c) RFLP d) Microarray
IV) _____________ technology uses radioactive probes to identify bands
derived from hyper variable regions of DNA.
a) DNA finger printing b) DNA foot printing
c) Colony hybridization d) In-situ hybridization
V) One of the application of site-directed mutagenesis is _____________
a) Microarray b) Microinjection
c) Protein engineering d) Gel matching
SLR-VR – 23 -2-
VI) ____________ method is used as a chemical method for protein sequencing.
a) Immunohistochemical b) Edman degradation
c) Di-deoxysangers d) Maxam and Gilbert
VII) Two-dimensional polyacrylamide gel electrophoresis (2-D -PAGE) is a
method used to separate proteins according to _____________
a) Centrifugal force b) Mass
c) Charge d) Charge and mass
VIII) The most commonly used general protein stain for detecting proteins on
gels is _____________
a) Ethidium Bromide
b) Methylene Blue
c) Coomassie Brilliant Blue
d) Alcian Blue
IX) _____________ chromatography method is used to separate a protein
that binds strongly to its substrate.
a) Gel filtration b) Cation exchange
c) Anion exchange d) Affinity
X) A commonly employed first separation step in protein isolation is ________
a) Ammonium sulfate precipitation
b) Gel filtration chromatography
c) Ion-exchange chromatography
d) Electrophoresis
B) Answer the following : 10
a) Taq polymerase
b) Probes
c) Expasy
d) Ampholytes
e) Dialysis.
-3- SLR-VR – 23
PART – II
Answer any four of the following :
2. Discuss the principles, methodology and applications of PCR. 20
3. Discuss the isolation and purification of proteins using chromatographic methods. 20
4. What are probes ? Elaborate principle, methodology and applications of Southern
blotting. 20
5. Write short answer (any two) : 20
a) Compare SDS-PAGE and Native-PAGE.
b) Colony hybridization and its application ins screening of recombinants
c) Write note on DNA fingerprinting.
6. Write short notes on (any four) : 20
a) Site directed mutagenesis
b) RFLP
c) 2-D PAGE
d) HPLC
e) Autoradiography
f) Edman degradation.
_____________
M.Sc. (Part – II) (Semester – IV) Examination, 2014
BIOINFORMATICS
Advanced Molecular Biology (Paper – III)
Day and Date : Saturday, 26-4-2014 Total Marks : 100
Time : 3.00 p.m. to 6.00 p.m.
Instructions :1) Part – I, question 1 is compulsory.
2) Attempt any four questions from Part – II.
3) Figures to the right indicate full marks.
4) Answers to the Part – I and Part – II are to be written in
same answer book only.
PART – I
1. A) Choose the correct answer from given alternatives : 10
I) The thermostable form of DNA polymerase used in PCR cycles is
_____________
a) Paq b) Baq c) Taq d) Maq
II) __________ blotting technique involves transfer of RNA molecules onto
membranes for the detection of specific sequences by hybridization.
a) Northern blotting b) Southern blotting
c) Western blotting d) South Western blotting
III) Relative expression levels of thousands of genes can be visualized
using _____________ technology.
a) Autoradiography b) DNA fingerprinting
c) RFLP d) Microarray
IV) _____________ technology uses radioactive probes to identify bands
derived from hyper variable regions of DNA.
a) DNA finger printing b) DNA foot printing
c) Colony hybridization d) In-situ hybridization
V) One of the application of site-directed mutagenesis is _____________
a) Microarray b) Microinjection
c) Protein engineering d) Gel matching
SLR-VR – 23 -2-
VI) ____________ method is used as a chemical method for protein sequencing.
a) Immunohistochemical b) Edman degradation
c) Di-deoxysangers d) Maxam and Gilbert
VII) Two-dimensional polyacrylamide gel electrophoresis (2-D -PAGE) is a
method used to separate proteins according to _____________
a) Centrifugal force b) Mass
c) Charge d) Charge and mass
VIII) The most commonly used general protein stain for detecting proteins on
gels is _____________
a) Ethidium Bromide
b) Methylene Blue
c) Coomassie Brilliant Blue
d) Alcian Blue
IX) _____________ chromatography method is used to separate a protein
that binds strongly to its substrate.
a) Gel filtration b) Cation exchange
c) Anion exchange d) Affinity
X) A commonly employed first separation step in protein isolation is ________
a) Ammonium sulfate precipitation
b) Gel filtration chromatography
c) Ion-exchange chromatography
d) Electrophoresis
B) Answer the following : 10
a) Taq polymerase
b) Probes
c) Expasy
d) Ampholytes
e) Dialysis.
-3- SLR-VR – 23
PART – II
Answer any four of the following :
2. Discuss the principles, methodology and applications of PCR. 20
3. Discuss the isolation and purification of proteins using chromatographic methods. 20
4. What are probes ? Elaborate principle, methodology and applications of Southern
blotting. 20
5. Write short answer (any two) : 20
a) Compare SDS-PAGE and Native-PAGE.
b) Colony hybridization and its application ins screening of recombinants
c) Write note on DNA fingerprinting.
6. Write short notes on (any four) : 20
a) Site directed mutagenesis
b) RFLP
c) 2-D PAGE
d) HPLC
e) Autoradiography
f) Edman degradation.
_____________
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