2014 Question Paper,B.Sc. II (Semester – IV),Solapur University Question Paper,BIOTECHNOLOGY Genetics II (Microbial Genetics)

Solapur University Question Paper
B.Sc. II (Semester – IV) Examination, 2014
BIOTECHNOLOGY
Genetics II (Microbial Genetics)
Day and Date : Thursday, 24-4-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions: 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Rewrite the following by selecting right alternative : 10
i) Plasmid is an extrachromosomal _____________ DNA molecule present in
most bacteria.
a) linear b) supercoiled
c) circular d) fibrilar
ii) A plasmid capable of preparing its own DNA for transfer is called
a) conjugative b) transmissible
c) nontransmissible d) mobilizable
iii) ____________ type of mutation involves replacement of purine by another
purine.
a) deletion b) transition
c) transversion d) inversion
iv) Recombination frequency is determined by ___________ in two genes.
a) similarity b) difference
c) distance d) dissimilarity
v) 5 BU (5-bromouracil) is an analog of ____________ base.
a) Uracil b) Adenine
c) Thymine d) Cytasine
vi) ____________ phage mediates generalised transduction.
a) λ b) Mu c) P22 d) T4
Seat
No.
SLR-D – 25 -2- 
vii) Acridine dyes lead to ___________ to cause mutation.
a) inversion b) reversion
c) intercalation d) transposition
viii) Is element contain coding sequences that are initiated by ____________
codon.
a) GUG b) AUG c) UAA d) UAG
ix) ____________ test was discoverd by Luria and Delbruck to prove spontaneous
nature of mutation.
a) Cis-trans b) Chi square
c) Fluctuation d) Hybridization
x) An exposure of bacteria to (EMS) ethylmethylsulfonate causes ________ of
purines.
a) alkylation b) amination
c) acetylation d) deamination
2. Answer any five of the following : 10
i) Write properties of a vector.
ii) Describe effects caused by nitrous acid.
iii) Write about lethal mutations.
iv) What is a partial diploid ? Write about its formation.
v) Explain ‘Generalised transduction’.
vi) Elaborate on structure of transposon.
3. A) Answer any two of the following : 6
i) Give an account of mutations caused by acridine dyes.
ii) Describe structure and properties of R plasmid.
iii) Describe composite transposons.
B) Give an account of mutations caused by ultraviolet radiations. 4
4. Answer any two of the following : 10
i) Write on transfer of plasmid.
ii) Describe bacterial conjugation.
iii) Describe replica plate technique.
5. Answer any two of the following : 10
i) Give an account of complementation analysis.
ii) Describe replicative transposition.
iii) Write on isolating of plasmids.
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