BIOENERGETICS AND METABOLISM – I,2014 Question Paper,B.Sc. – II (Biotechnology) (Semester – III),Solapur University Question Paper

Solapur University Question Paper
B.Sc. – II (Biotechnology) (Semester – III) Examination, 2014
BIOENERGETICS AND METABOLISM – I
Bioenergetics and Thermodynamics
Day and Date : Monday, 26-5-2014 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
 N.B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw neat labelled diagram wherever necessary.
1. Rewrite the sentences by choosing correct alternative given below : 10
i) The reaction is said to be at equilibrium when its actual free energy change is
_________________
a) Negative b) Positive c) Zero d) One
ii) For spontaneous reactions the sum of entropy of system and surrounding is
always _________________
a) > 0 b) < 0 c) = 0 d) ≤ 0
iii) The electron donating molecule in an oxidation reduction reaction is called
as __________________
a) Oxidant b) Reductant
c) Oxidizing agent d) Both a) and c)
iv) ________________ coenzyme is involved in decarboxylation reactions of
α -keto acids.
a) Tetrahydrofolate b) Cobamide
c) NAD+ d) TPP
v) ________________ is protease enzyme which specifically cleaves carboxy
terminal amino acid.
a) Chymotrypsin b) Carboxy peptidase
c) Lysozyme d) All of these
SLR-D – 23 -2- 
vi) ________________ inhibitor can bind only to free enzyme not to the enzymesubstrate
complex.
a) Competitive b) Uncompetitive
c) Non-competitive d) Mixed
vii) The bell shaped curve is obtained for effect of ________________ on enzyme
activity.
a) Enzyme concentration b) Substrate concentration
c) Temperature d) All of these
viii) The standard free energy change of hydrolysis of ATP to ADP is
________________ Kcal/mole.
a) – 35 b) – 30.5 c) – 15.7 d) – 7.3
ix) The relationship between free energy, enthalpy and entropy is given by
________________
a) Δ G = Δ H – TΔ S b) Δ H = Δ G – TΔ S
c) ΔS = Δ G – TΔ H d) ΔS = Δ H – TΔ G
x) Among the following ________________ has highest group transfer potential.
a) 1,3-Bisphosphoglycerate
b) Creatinine phosphate
c) Carbonyl phosphate
d) Phosphoenol pyruvate
2. Answer any five of the following : 10
i) Give second law of thermodynamics.
ii) State first order reaction. What is its kinetic ?
iii) Define Katal the unit of enzyme activity.
iv) What are high energy phosphate compound ? Mention two examples.
v) Explain coupled reaction.
vi) What is oxidation in terms of electron ? Give one example.
 -3- SLR-D – 23
3. A) Answer any two of the following : 6
i) Give the applications of thermodynamics in biochemistry.
ii) What is coenzyme ? What is the role of TPP and FAD as coenzyme ?
iii) Explain the hydrolysis of ATP.
B) Describe in detail the effect of substrate concentration on enzyme activity. 4
4. Answer any two of the following : 10
i) Give the significance of Km. Derive line weaver Burk’s equation and plot it.
ii) What is standard free energy change ? How it is determined for a reaction ?
iii) What is non-competitive inhibitor ? Give its effect on Km and Vmax of enzymatic
reaction.
5. Answer any two of the following: 10
i) What is redox potential ? Explain in detail how it is measured.
ii) Discuss in detail the mechanism of action of enzyme lysozyme.
iii) How covalent catalysis, stain and distortion theory involved in enzyme
efficiency ?
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