B.Sc. – I (Sem. – II),2014 Question Paper,BASIC BIOTECHNOLOGY – II (Old),Solapur University Question Paper

Solapur University Question Paper
B.Sc. – I (Sem. – II) (Biotechnology) Examination, 2014
BASIC BIOTECHNOLOGY – II (Old)
Day and Date : Monday, 12-5-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
 N.B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw neat and labelled diagrams wherever necessary.
1. Rewrite the following sentences by choosing correct alternatives given below : 10
1) Variants selected in protoplast cultures have been referred to as _________
a) Calliclones b) Protoclones c) Polyclones d) Aneoclones
2) Over population of ______________ sometimes enhance the protein value
of the variant cell lines.
a) Auxins b) Cytokinins c) Amino acid d) Antibodies
3) Somaclonal variations are the ones __________
a) caused by mutagens
b) caused by hybridization
c) Produced during tissue culture
d) Induced during sexual embryogeny
4) A GM crop is __________
a) Irradiated crop b) Transgenic crop
c) Raised on green manure d) Wild crop
5) _____________ enzymes are often used for isolation of protoplast.
a) Gyrase b) Polymerase c) Cellulase d) Invertase
6) The organism used for production of ‘BT’ bioinsecticide belongs to the
genus ___________
a) Borrelia b) Bacillus c) Bordetella d) All of these
7) _____________ is novel technique involving combination of RFLP and RAPD.
a) QTL b) AFLP c) SCAR’s d) STS
SLR-D – 13 
8) Bt cotton is preferred over other varieties of cotton because of its ______
a) High yield b) Quick growth
c) Weedicidal property d) Resistance to bollworm
9) Virus free plants can be obtained through _________
a) Shoot tip culture b) Haploid culture
c) Proto plast fusion d) Embryo culture
10) __________ preservation of cell suspension was considered as a potential
method for plant germplam storage.
a) Freeze drying b) Cold storage
c) Liquid N2 freeze d) Freezing
2. Answer any five of the following : 10
i) Somoclones ii) Hybrids
iii) Protoplast iv) Germ-plasm
v) Pigments vi) Cloning.
3. A) Answer any two of the following : 6
i) Write a note on protoplast culture.
ii) Give an account of herbicide resistant plant.
iii) Describe mechanism of aided breeding.
B) Explain how can we produce male sterile plants. 4
4. Answer any two of the following : 10
i) Explain the mechanism of isolation of stress tolerant line.
ii) Give detailed account of cytopreservation.
iii) Describe method of production of plant resistant to pathogens.
5. Answer any two of the following : 10
i) How can we improve time of flowering in plants ?
ii) Explain RFLP on a molecular marker.
iii) Write a note on molecular marker assisted selection.
_____________________

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