B.Sc. – I (Semester – I),2014 Question Paper,BIOTECHNOLOGY (Old) Basic of Tissue Culture – I,Solapur University Question Paper

Solapur University Question Paper
B.Sc. – I (Semester – I) Examination, 2014
BIOTECHNOLOGY (Old)
Basic of Tissue Culture – I
Day and Date : Saturday, 7-6-2014 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
 N.B. : 1) All questions are compulsory.
2) Figures to right indicate full marks.
3) Draw neat labelled diagrams wherever necessary.
1. Rewrite the following sentences by using correct alternative : 10
1) Which of the following plant cell show totipotency ?
a) Xylem vessel b) Sieve tube
c) Meristem d) Cork cells
2) To obtain triploid plant we culture
a) Nucleus b) Anther
c) Endosperm d) Embryo
3) _____________ hormone is used to obtain roots in tissue culture.
a) Auxin b) Cytokinin
c) Gibberellin d) Ethylene
4) In animal cell culture, the addition of serum to media is essential for
providing _____________
a) Amino acids for protein synthesis
b) Nucleotides for DNA synthesis
c) Growth factors
d) All of these
SLR-D – 5 -2-
5) To promote attachment and spreading of anchorage dependent animal cells,
the surface of the culture vessel needs to be coated with
a) Trypsin b) Cellogen
c) Pronase d) Polyglycol
6) In animal cell culture hormone FGF stands for
a) Filamentous growth factor b) Fibroblast growth factor
c) Fibronectin growth factor d) Fetuin growth factor
7) Monoclonal antibodies are produced by
a) Invivo method
b) Suspended cell culture in fermentor
c) Mobilized cell reactors
d) All of these
8) The major hazards of monoclonal antibodies are
a) .Difficult in purification
b) Contamination with retroviral particles from mouse myeloma cells
c) Non specificity
d) All of these
9) Which of these would not be used to introduce DNA in animal cells ?
a) Liposomes b) Electroporation
c) Microinjection d) T1 plasmid
10) Anchorage dependent means
a) Attachment to the substrate
b) Attachment to cell
c) Attachment to nutrients
d) Attachment to nucleus
-3- SLR-D – 5
2. Attempt any five of the following : 10
1) What is embryo ?
2) Define cloning.
3) Viability.
4) Use of cell culture.
5) Define callus.
6) Define hybrids.
3. a) Attempt any two of the following : 6
1) Give an account on cytodifferentiation.
2) Explain suspension culture.
3) What is cell transformation ?
b) Explain embryo transplant technique. 4
4. Answer any two of the following : 10
1) Explain the method of production of novel plants.
2) Illustrate the preparation of plant tissue culture media.
3) Explain measurement of cell viability.
5. Answer any two of the following : 10
1) Explain production of monoclonal antibodies.
2) Describe invitro fertilization.
3) Explain method of developing transgenic animal.
_____________________

Share This