B.Sc. – II (Entrepreneurship) (Semester – IV),MICROBIOLOGY AND BIOTECHNOLOGY (Paper – VII),Solapur University Question Paper,2014 Question Paper

Solapur University Question Paper
B.Sc. – II (Entrepreneurship) (Semester – IV) Examination, 2014
MICROBIOLOGY AND BIOTECHNOLOGY (Paper – VII)
Molecular Biology
Day and Date : Tuesday, 29-4-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
1. Rewrite the sentences by selecting the correct answer from the given
alternatives.
1) Semiconservative DNA replication was first demonstrated by ___________
a) Taylor b) Watson and Crick
c) Meselson d) Khorana
2) During DNA replication the strands separated by ___________
a) DNA polymerase b) Gyrase
c) DNA helicase d) DNA primase
3) During DNA replication the term leading strand is applied to the one which
replicates in ___________
a) 5′ − 3′ direction continuously
b) 3′ − 5′ direction continuously
c) 5′ − 3′ direction discontinuously
d) 3′ − 5′ direction discontinuously
4) Genetic code is a sequence of nitrogenous bases on ___________
a) mRNA b) tRNA c) rRNA d) DNA
5) The terminator codon are ___________
a) UAA, UAG, UGA b) AUG, UAG, UGA
c) UAC, AUG, UAG d) AUG, ACG, GAG
6) A codon consist of how many nitrogen bases in it
a) 1 b) 2 c) 3 d) 4
7) Transcription is the transfer of genetic information from ___________
a) Chromosome to cytoplasm b) tRNA to mRNA
c) DNA to mRNA d) mRNA to rRNA
SLR-E – 29
8) Transcription takes place in ___________
a) Matrix b) Nucleus c) Cytosol d) Cytoplasm
9) During transcription the DNA site at which RNA polymerase binds is
called ___________
a) Promoter b) Regulator c) Receptor d) Enhancer
10) The gene not expressing any protein is known as ___________
a) Epistatic gene b) Hypostatic gene
c) Pseudogene d) None of these
2. Answer any five of the following : 10
a) Types of RNA polymerases and its functions.
b) Role of interferon gene regulation in eukaryotes.
c) Process of translation.
d) Properties of genetic code.
e) Attenuation.
f) Restriction maps.
3. A) Answer any two of the following : 6
a) Significance of RFLP.
b) Overlapping genes.
c) r-DNA processing.
B) Write the answer of following. 4
Gene regulation in prokaryotes.
4. Answer any two of following : 10
a) Give detail account of types of DNA repair system.
b) Write in detail SOS repair mechanism.
c) Write in detail fine structure of gene.
5. Answer any two of following : 10
a) Maxum and Gilbert method.
b) Gene regulation in bacteriophages.
c) Explain in detail ‘conjugation process’.
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