B.Sc. – II (Sem. – III),BIOTECHNOLOGY Molecular Biology – I,Solapur University Question Paper,2014 Question Paper

Solapur University Question Paper

B.Sc. – II (Sem. – III) Examination, 2014

BIOTECHNOLOGY

Molecular Biology – I (Molecular Biology of Gene)

Day and Date : Thursday, 22-5-2014 Max. Marks : 50

Time : 3.00 p.m. to 5.00 p.m.

 N.B. : 1) All questions are compulsory.

2) Figures to right indicates full marks.

1. Rewrite the following sentences by selecting correct answer from the given

alternatives. 10

1) Split gene start and end with _______________

a) Intron b) muton c) recon d) exon

2) _____________ plays important role in photoreactivation.

a) photolyase b) Exonuclease

c) endonuclease d) methylase

3) In A – DNA – base pairs are present in per turn of the helical.

a) 10 b) 11 c) 12 d) 13

4) A nucleoid consists of ______________

a) a ss DNA b) a single double stranded DNA

c) histones and RNA d) histones and nonhistones

5) Centromere is also called ______________

a) Kinetochore b) Chromocentre

c) Secondary constriction d) Kinetosome

6) Triplet codon refers to sequence of three bases on ____________

a) mRNA b) t RNA c) rRNA d) all the above

7) Histone protein synthesis occurs during ______________

a) G1 – phase b) G2 – phase c) S - phase d) Prophase

8) RNA does not possess ____________

a) Uracil b) Thymine c) Adenine d) Cytosine

SLR-D – 20 

9) Okazaki fragments are ________________

a) Small segments of RNA

b) Small peptides

c) Small DNA fragments

d) Small DNA fragments formed over DNA template running in 3′ → 5′ direction

10) t RNA attaches amino acids at its _______________

a) 3′ end b) 5′ end c) anticodon d) loop

2. Answer any five of the following : 10

1) What are mini satellites ?

2) Define – Introns

3) Define – Gene

4) What is histone ?

5) Enlist the properties of B-DNA from.

6) What is cot curve ?

3. A) Answer any two of the following : 6

1) Explain the organization the viral genome.

2) Give an account of molecular nature of gene.

3) What is SOS repair mechanism ?

B) Write an essay on – structure and functions of t RNA. 4

4. Write short notes on any two of the following : 10

1) DNA damage

2) Euchromatin

3) Mechanism of DNA replication in prokaryotes.

5. Write short notes on any two of the following : 10

1) Genetic code

2) Nucleotide excision repair

3) Regularity sequences.

_____________________

Share This