B.Sc. (Part – III) (Semester – VI),2014 Question Paper,MICROBIOLOGY Special Paper – XIII : Microbial Genetics,Solapur University Question Paper

Solapur University Question Paper
B.Sc. (Part – III) (Semester – VI) Examination, 2014
MICROBIOLOGY
Special Paper – XIII : Microbial Genetics
Day and Date : Thursday, 10-4-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Rewrite the sentences by choosing correct answers from given alternatives. 10
i) Site directed Oligonucleotide mutagenesis is commonly employed in
a) Gene mapping b) Gene sequencing
c) Protein Engineering d) DNA finger printing
ii) ___________ vector possess centromeric and telemeric regions.
a) Yacs b) BACS c) shuttle d) λ phage
iii) The Folded fibre model of E-coli chromosomes was proposed by
a) Pettijohn and Hetcht b) John Cains
c) Worcel d) Hershy and Chase
iv) When two mutations in a region of DNA cause negative Cis-trans test, that
region is called as
a) Muton b) Cistron c) Gene d) Replicon
v) DNA fragments are joined by _______ enzyme in DNA replication.
a) DNA polymerase I b) DNA polymerase II
c) DNA polymerase III d) DNA ligase
vi) __________ enzyme can add phosphate group from ATP to 5' OH end of DS
or SS DNA.
a) Alkaline phosphatase b) Acid phosphatase
c) Polynucleotide kinase d) Exonuclease
vii) During transcription synthesis of mRNA takes place in ______ direction.
a) 3′ → 5′ b) 5′ → 3′
c) 5′ → 3′ and 3′ → 5′ d) Any P.T.O.
Seat
No.
SLR-C – 164 -2-
viii) A base pair substitution that changes codon specific for one amino acid to
codon specific for another amino acid is called ___________ mutation.
a) Silent b) Missense
c) Non Sense d) Neutral
ix) ECORI enzyme is obtained from E. coli strain
a) RI b) Ry 12 c) Ry 13 d) Ry 1
x) Synthesis of galactosidase permease in E. coli in controlled by __________
gene.
a) lac O b) lac Z c) lac Y d) Lac a
2. Answer in 2-3 sentences any five of the following. 10
i) Write briefly on Plasmid
ii) Write briefly on pTi
iii) Give brief account of replacement vector
iv) What are Okazaki fragments ?
v) Write briefly on ‘Microprojectile’.
vi) What are suppressor mutations ?
vii) Write briefly on Palindrome sequence.
3. A) Answer any two of the following. 6
i) Write briefly on Nonsense mutation.
ii) Draw a neat labelled diagram of pBR 322.
iii) Draw a diagram showing structure of Lac Operon.
B) Write short note on, ‘Application of Protein Engineering’. 4
4. Answer any two of the following. 10
i) Describe briefly folded fibre model of E. coli chromosome.
ii) Describe briefly different methods used for selection of recombinant clones.
iii) Describe briefly process of Transcription.
5. Answer any two of the following. 10
i) Describe briefly the enzymes involved in DNA replication.
ii) Discuss briefly ‘Cis-Trans Test’.
iii) Give brief account of DNA finger printing.
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