MICROBIOLOGY – II (Section – I) (Old),Solapur University Question Paper,B.Sc. – I (Sem. – II),2014 Question Paper

Solapur University Question Paper
B.Sc. – I (Sem. – II) (Biotechnology) Examination, 2014
MICROBIOLOGY – II
 (Section – I) (Old)
Day and Date : Thursday, 15-5-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
 N.B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Rewrite the following sentences by selecting correct answer from given
alternatives : 10
1) Root nodules of the leguminous plants contain _______________
a) Rhizobium b) Azotobacter
c) Bacillus thuringiensis d) Pseudomonas
2) ______________ is a primary stain in, acid fast staining of mycobacteria.
a) Crystal violet b) Carbol fuchsin
c) Methylene blue d) Basic fuchsin
3) Heat labile solutions can be sterilized by ____________
a) autoclaving b) lyophilisation
c) filtration d) none of these
4) _____________ is an example of dual fermentation.
a) Alcohol b) Beer
c) Wine d) Vinegar
5) ____________ are present in fermentor to avoid vortex formation.
a) Baffle b) Sparger
c) Impeller d) None of these
6) ______________ is an example of secondary metabolite produced by
microorganisms.
a) Citric acid b) Ethanol
c) Penicillin d) Vitamin B12
SLR-D – 15 
7) _______________ classification system is based on evolutionary relationship.
a) Phylogenetic b) Phenetic
c) Numerical d) Classical
8) Lab scale fermentor have ______________ liters of capacity.
a) 1 – 2 b) 50 – 100
c) 20 – 50 d) 1000 – 5000
9) ______________ is an example of endoenzyme produced by microorganisms.
a) Lipase b) Amylase
c) Protease d) Invertase
10) _____________ is a variant bacterial strain with distinctive antigenic properties.
a) Morphovar b) Biovar
c) Serovar d) Pathovar
2. Answer any five of the following : 10
1) Define – Sterilization.
2) Define – Neutral stain. Give any two examples.
3) Define – type strain.
4) Give the recommended uses of hot air oven.
5) Define – secondary metabolites. Give any two examples.
6) What are antifoam agents ?
3. A) Answer any two of the following : 6
1) Write a note of negative staining.
2) Give an account of nonionizing radiations for control of microorganisms.
3) Write a note on recovery of fermentation product.
B) Write a note on acid fast staining. 4
4. Write short notes on any two : 10
1) Morphological characters used for classification.
2) Microbial enzymes.
3) Moist heat sterilization.
5. Write short notes on any two : 10
1) Biogas production.
2) Basic design of fermentor.
3) Genetic improvement of industrial microbes.
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