Solapur University Question Paper MICROBIOLOGY Paper-v 2014 Question Paper

Solapur University Question Paper M.Sc. (Part – I) (Semester – II) Examination, 2014 (New C.G.P.A. Pattern) MICROBIOLOGY (Paper – V) Microbial Genetics Day and Date : Tuesday, 22-4-2014 Max. Marks : 70 Time : 11.00 a.m. to 2.00 p.m. N.B. : 1) Part – I question 1 is compulsory. 2) Attempt any four questions from Part – II. 3) Figures to the right indicates full marks. 4) Answer to the two Parts should be written in the same answer book. PART – I 1. Rewrite the sentences after choosing the correct answer from the given alternatives. 14 1) In A form of DNA, one turn of helix consists of ___________ base pairs. a) 10 b) 11 c) 9.33 d) 8 2) Ovalbumin gene in chickens is an example of ___________ a) Satellite DNA b) Split gene c) Palindromic DNA d) Relaxed DNA 3) One cistron one polypeptide hypothesis was developed by ___________ a) Beadle and Tatum b) Ochoa c) Lederberg d) Delbruck 4) Mendel for his experimental work used ___________ plan. a) Groundnut b) Garden pea c) French bean d) Soyabean 5) Amplification of plasmids is carried out by _____________ a) Penicillin b) Streptomycin c) Chloramphenicol d) Tetracyclin SLR-VO – 5 -2- 6) In folded fiber model of E.coli chromosome, DNA folds are held by __________ a) DNA b) RNA c) Proteins d) Enzymes 7) Transposon Tn3 carries gene for ____________ resistance. a) Tetracyclin b) Ampicillin c) Penicillin d) Chloramphenicol 8) Photo-reactivation is used to repair ____________ types of damage. a) Double stranded breaks b) Single stranded breaks c) T-T Dimmers d) Point mutation 9) __________ sponsors conjugation in bacteria. a) F plasmid b) R plasmid c) Chromosomal DNA d) Ti plasmid 10) __________ is the initiation codon during translation. a) AUG b) UUU c) UAG d) CCC 11) ___________ is the initiator t-RNA in protein synthesis. a) t-Met-tRNAt b) a-Met-tRNAa c) v-Met-tRNAv d) f-Met-tRNAf 12) Peptidyl t-RNA occupies ___________ site in the ribosome. a) A b) E c) P d) Q 13) ____________ is the transforming principle taken up by bacteria in Griffith’s experiment. a) RNA b) Polypeptide c) Carbohydrate d) DNA 14) ___________ causes point mutation in genome. a) EMS b) UV radiation c) Gamma radiation d) Visible light -3- SLR-VO – 5 PART – II 2. Explain the roles of different enzymes involved in the E.coli DNA replication. 14 3. Discuss in detail the process of transcription in prokaryotic organisms. 14 4. Explain types, properties and mechanism of transposition of transposable element.14 5. Attempt any two of the following : 14 a) Explain the technique and applications of PCR. b) Justify the statement “The genetic code is deciphering.” c) Describe operon model with reference to lactose operon. 6. Write short notes on any two of the following : 14 a) Alternative forms of DNA b) Overlapping genes c) LCR. _____________________

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