Solapur University Question Paper ,MICROBIOLOGY Biophysics and Bioinstrumentation (Paper – VII), 2014 Question Paper

Solapur University Question Paper M.Sc. – I (Semester – II) Examination, 2014 MICROBIOLOGY (New) (C.G.P.A. Pattern) Biophysics and Bioinstrumentation (Paper – VII) Day and Date : Saturday, 26-4-2014 Max. Marks : 70 Time : 11.00 a.m. to 2.00 p.m. N.B. : 1) Part – I question 1 is compulsory. 2) Attempt any four questions from Part – II. 3) Figures to the right indicate full marks. 4) Answer to the two Parts should be written in the same answer book. PART – I 1. Rewrite the following sentence by selecting the correct answer from given alternatives. 14 1) In X-ray tube ______________ is used as electron source. a) Anode b) Cathode c) Grid d) Lead 2) Optical rotation measurement is denoted by letter ____________ a) β b) γ c) α d) φ 3) The source of wavelength used in Raman spectroscopy is _____________ a) Mercury lamp b) Cathod lamp c) Tungsten lamp d) u.v. lamp 4) In u.v. – VIS spectroscopy the source of u.v. light is ___________ lamp. a) Tungsten b) Deuterium c) Hydrogen d) Mercury 5) In mass spectrometry separation of molecule is based on their ____________ a) Mass b) Charge c) Mass to charge ratio d) Length SLR-VO – 7 -2- 6) First step in atomic absorption spectroscopy is ___________ a) Ionization b) Chemical treatment c) Sublimation d) Nebulization 7) Northern blotting technique is used for detection of ____________ a) DNA b) RNA c) Protein d) Carbohydrate 8) 3H is weak ______________ a) γ -emitter b) α -emitter c) electron d) β-emitter 9) The glass electrode contain ___________ solution. a) 0.1 M HCl b) 1M HCl c) 1M KCl d) 10 mM HCl 10) Combined electrode consist of _______________ a) glass and reference electrode b) glass electrode c) reference electrode d) mercury electrode 11) Tertiary structure of protein is maintained by ____________ a) peptide bond b) hydrogen bond c) di-sulphide bond d) all of the above 12) Which of the following technique involves the separation of antigen based on their sizes and electrical charges ___________ a) radial immunodiffusion b) immuno electrophoresis c) agglutination d) immuno filtration 13) _____________ are amplified in photomultiplier tube. a) photon b) electron c) proton d) positron 14) 35s and 14c are weak ____________ a) α -emitters b) β -emitters c) γ -emitters d) positron -3- SLR-VO – 7 PART – II Attempt any four questions : 2. Explain the principle, instrumentation and application of X-ray crystallography. 14 3. Why atom shows radio activity ? Take detailed account of autoradiography. 14 4. What is Beer and Lamberts Law ? Explain the principle, and working of u.v. – VIS spectrometer. 14 5. Write short answer (any two) : 14 a) Explain the principle and working of pH meter. b) Describe the chemical properties of amino acid and proteins. c) Explain the principle and application of fluorescence immuno assay. 6. Write short note on (any two) : 14 a) Circular dichroism. b) Compare and contrast turbidometry and nephalometry. c) 3D structure determination by X-ray diffraction. _____________________

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