MICROBIOLOGY (Paper – IX) 2014 QuestionPA Solapur University Question Paper

Solapur University Question Paper M.Sc. (Part – II) (Semester – III) Examination, 2014 MICROBIOLOGY (Paper – IX) Molecular Biology and Genetic Engineering Day and Date : Monday, 21-4-2014 Max. Marks : 100 Time : 3.00 p.m. to 6.00 p.m. N. B. : 1) Part – I question – 1 is compulsory. 2) Attempt any four questions from Part – II. 3) Figures to the right indicate full marks. 4) Answers to the two Parts should be written in the same answer book. PART – I 1. Rewrite the following sentences by selecting the correct answer from given alternatives : 20 i) The spontaneous nature of mutation in bacteria was discovered by _____________ a) Lederberg b) Tatum c) Luria and Delbruick d) Watson ii) _____________ is an example of physical mutagen. a) NTG b) Acridine dyes c) ETBr d) U.V. iii) The transformation in bacteria was discovered by _____________ a) Griffith b) Lederberg c) Tatum d) Zinder iv) _____________ introduced pBR series of plasmids. a) Bolliver and Rous b) Boyd and Rodriguez c) Bolliver and Rodriguez d) Bolliver and Roston v) _____________ is an example of ssDNA phage vector. a) T2 b) T4 c) M13 d) Lambda SLR-VO – 9 -2- vi) _____________ enzyme is used in construction of rDNA by homopolymer tailing method. a) Bet-galactosidase b) Terminal transferase c) Permease d) Protease vii) _____________ methodology is used in protein engineering technique. a) UV mutagenesis b) Site directed mutagenesis c) Transposon mutagenesis d) ETBr mutagenesis viii) In the pUC series of plasmid vectors ‘UC’ stands for _____________ a) Uni of Cuttak b) Union Carbide c) Uni of Canada d) Uni. of California ix) _____________ is an example of biological mutagen. a) Tn-5 b) ETBr c) NTG d) 5Bru x) Mutagenicity test is known as _____________ test. a) Elek b) Widal c) Ames d) Weil-Felix xi) The fluctuation test was performed by _____________ a) Luria and Delbruck b) Lederberg c) Mullis d) Maxam xii) Bacterial conjugation was discovered by _____________ a) Beadle b) Griffith c) Lederberg ad Tatum d) Zinder xiii) _____________ is not an example of site specific restriction enzyme. a) EcoRI b) Hindiii c) BamHi d) EcoK xiv) Use of _____________ is an example of non-radioactive labeling of DNA. a) C14 b) N15 c) H3 d) Avidin-Biotin system xv) The Dideoxy method of DNA sequencing was devised by _____________ a) Sanger b) Crick c) Maxam and Gilbert d) Mullis xvi) _____________ host is commonly used in genetic engineering. a) M. tuberculosis b) E. aerogenes c) E.coli-K12 X1776 d) S. typhi -3- SLR-VO – 9 xvii) PCR technique was developed by _____________ a) Kary Mullis b) Hershey c) Sanger d) Gilbert xviii) Signal hypothesis describes protein _____________ a) Sequencing b) Digestion c) Synthesis d) Transporting xix) Transduction was discovered by _____________ a) Griffith b) Zinder and Lederberg c) Tatum d) Crick xx) Nitrogenase enzyme converts N2 to _____________ a) NO2 b) NO c) NH3 d) NO3 PART – II Attempt any four questions : 2. What are genomic and cDNA libraries ? Compare and contrast in detail. 20 3. What is protein engineering ? Describe methodology and applications. 20 4. What is conjugation ? Describe important features of conjugation process and its significance. 20 5. Write short answers (any two) : 20 i) What is site directed mutagenesis ? Describe oligonucleotide based site directed mutagenesis.. ii) Describe with example different methods of construction of radiolabeled probes. iii) Restriction and modification and their significance. 6. Write short notes on (any four) : 20 i) pBR 322 plasmid vector ii) CsCl2 density gradient centrifugation iii) Nick translation iv) Neoplastic transformations v) C-value paradox vi) Homopolymer tailing technique. _____________

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