Solapur University Question Paper,INDUSTRIAL CHEMISTRY Paper – VII : Techniques in Industrial Chemistry,2014 Question Paper ,B.Sc. (Part – II) (Semester – IV)

Solapur University Question Paper
B.Sc. (Part – II) (Semester – IV) Entrepreneurship Examination, 2014
INDUSTRIAL CHEMISTRY
Paper – VII : Techniques in Industrial Chemistry
Day and Date : Saturday, 26-4-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
3) Draw neat and labelled diagrams wherever necessary.
1. Choose the most correct alternative from among those given below : 10
1) Spectroscopic methods are advantageous because they are _____________
a) Useful for the determination of unknown structures
b) Rapid methods for analysis
c) Sensitive, accurate and highly reliable methods
d) All above
2) _____________ is a method of elemental analysis.
a) AAS b) Chromatography
c) Electrophoresis d) None of these
3) In flame photometeres, the detectors used are _____________
a) Thermopiles b) Photomultipliers
c) Tungsten tubes d) Colorimeteres
4) X-ray diffraction technique uses _____________ law for crystallographic
analysis.
a) Lambert’s b) Beer’s
c) Bragg’s d) Partition
5) _____________ is the cross-linked dextran used as popular gel for separation
of proteins and other biomolecules.
a) Sephadex b) Quartz
c) Agarose d) Starch
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6) Gel permeation chromatography can be performed by ______ chromatography.
a) Paper b) Gas
c) Ion-exchange d) Column or thin layer
7) _____________ method is used for separation and characterisation of proteins
based on electrophoresis and reaction with antibodies.
a) Gel filtration chromatography
b) Immunoelectrophoresis
c) SDS-PAGE
d) AAS
8) To determine crystal structure of material _____________ technique is used.
a) Chromatography
b) SDS-PAGE
c) Photometry
d) X-ray diffraction
9) In SDS-PAGE _____________ is used for determination of molecular weights
of proteins.
a) Polyacrylamide b) Agarose
c) CSDS d) Starch
10) The sensitivity in AAS is generally expressed in terms of _____________
for 1% absorbance.
a) μg/ml b) mg/ml
c) g/ml d) g/dm3
2. Answer any five of the following : 10
i) Explain the term absorption spectroscopy.
ii) What are nebulizers ?
iii) State applications of X-ray diffraction methods to complexes.
iv) Explain the term chromatography.
v) Write applications of gel chromatography.
vi) Write the types of electrophoresis.
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3. A) Answer any two of the following : 6
i) Explain the function of photomultiplier tube used in AAS.
ii) What is elastic scattering ?
iii) Why should electrophoresis be done in solutions of low salt concentrations ?
B) Explain the process of SDS-PAGE. 4
4. Answer any two of the following : 10
i) Give a brief account of mirrors and slits used in flame photometry.
ii) Discuss the technique of gel filtration chromatography.
iii) Explain the process of high resolution X-ray diffraction method.
5. Answer any two of the following : 10
i) Explain the functions of the components involved in atomic absorption
spectrophotometer.
ii) Write a note on immunoelectrophoresis.
iii) Explain the process of X-ray diffraction used in crystallographic analysis.
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