Solapur University Question Paper
B.Sc. – I (Semester – I) Examination, 2014
BIOTECHNOLOGY
Microbiology – I (Old)
Day and Date : Monday, 9-6-2014 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N.B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Rewrite the following sentences by selecting correct answer from given
alternatives : 10
1) In gram negative bacteria, the flagellum is attached to the cytoplasmic
membrane by the rings _____________
a) S ring only b) S and M rings
c) P ring d) P and L rings
2) _____________ discovered bacteria.
a) Leeuwenhoek b) Lederberg
c) Wolkman and Zinder d) Von Dusch
3) Bacteria differ from plants in that they do not have _____________
a) DNA b) RNA
c) Cell wall d) A well defined nucleus
4) When a pair of spherical bacteria is present together it is called
_____________
a) Streptococcus b) Micrococcus
c) Diplococcus d) Sarcina
5) Mac Conkey’s agar is an example of _____________
a) Living media b) Enriched media
c) Enrichment media d) Selective and differential media
6) HeLa cell line is an example of _____________
a) Continuous cell line b) Primary cell culture
c) Diploid cell culture d) None of the above
7) The type of ribosome present in a prokaryotic cell is _____________
a) 25 S b) 70 S c) 80 S d) 60 S
8) Germ theory of disease was given by _____________
a) Robert Koch b) Louis Pasteur
c) A. V. Leeuwenhoek d) L. J. Stadler
9) An increase in the _____________ is observed during lag phase of growth.
a) Cell No. b) Cell size c) Cell mass d) Growth rate
10) In turbidostat _____________ is kept as a predetermined value.
a) Growth rate b) Dilution rate
c) Turbidity d) Cell mass
2. Answer any five of the following : 10
1) Define – isolation.
2) Define – continuous culture.
3) Enlist the functions of capsule.
4) Enlist the various types of cytoplasmic inclusions.
5) Define – Fungi.
6) Define – geomicrobiology.
3. A) Answer any two of the following : 6
1) Enlist the differences between capsule and slime layer.
2) Draw a neat labelled diagram of prokaryotic cell.
3) Write a note on chemoheterotrophs.
B) Define – pure culture. Explain the various methods of selection of pure culture. 4
4. Write short notes on any 2 : 10
1) Contributions in development of microscope
2) Laboratory methods of determination of growth
3) Structure of Gram negative bacterial cell wall.
5. Write short notes on any 2 : 10
1) Reserve food materials
2) Streak plate method
3) Living media.
_______________
B.Sc. – I (Semester – I) Examination, 2014
BIOTECHNOLOGY
Microbiology – I (Old)
Day and Date : Monday, 9-6-2014 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N.B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Rewrite the following sentences by selecting correct answer from given
alternatives : 10
1) In gram negative bacteria, the flagellum is attached to the cytoplasmic
membrane by the rings _____________
a) S ring only b) S and M rings
c) P ring d) P and L rings
2) _____________ discovered bacteria.
a) Leeuwenhoek b) Lederberg
c) Wolkman and Zinder d) Von Dusch
3) Bacteria differ from plants in that they do not have _____________
a) DNA b) RNA
c) Cell wall d) A well defined nucleus
4) When a pair of spherical bacteria is present together it is called
_____________
a) Streptococcus b) Micrococcus
c) Diplococcus d) Sarcina
5) Mac Conkey’s agar is an example of _____________
a) Living media b) Enriched media
c) Enrichment media d) Selective and differential media
6) HeLa cell line is an example of _____________
a) Continuous cell line b) Primary cell culture
c) Diploid cell culture d) None of the above
7) The type of ribosome present in a prokaryotic cell is _____________
a) 25 S b) 70 S c) 80 S d) 60 S
8) Germ theory of disease was given by _____________
a) Robert Koch b) Louis Pasteur
c) A. V. Leeuwenhoek d) L. J. Stadler
9) An increase in the _____________ is observed during lag phase of growth.
a) Cell No. b) Cell size c) Cell mass d) Growth rate
10) In turbidostat _____________ is kept as a predetermined value.
a) Growth rate b) Dilution rate
c) Turbidity d) Cell mass
2. Answer any five of the following : 10
1) Define – isolation.
2) Define – continuous culture.
3) Enlist the functions of capsule.
4) Enlist the various types of cytoplasmic inclusions.
5) Define – Fungi.
6) Define – geomicrobiology.
3. A) Answer any two of the following : 6
1) Enlist the differences between capsule and slime layer.
2) Draw a neat labelled diagram of prokaryotic cell.
3) Write a note on chemoheterotrophs.
B) Define – pure culture. Explain the various methods of selection of pure culture. 4
4. Write short notes on any 2 : 10
1) Contributions in development of microscope
2) Laboratory methods of determination of growth
3) Structure of Gram negative bacterial cell wall.
5. Write short notes on any 2 : 10
1) Reserve food materials
2) Streak plate method
3) Living media.
_______________
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