Annamalai University B.Sc. (Botany):Biological techniques and tissue culture

Annamalai University question paper december 2014
B.Sc. DEGREE EXAMINATION, December 2014
(BOTANY)
(THIRD YEAR)
(PART-III)
750. BIOLOGICAL TECHNIQUES AND TISSUE CULTURE
Time: Three hours Maximum: 100 marks
 SECTION – A
 Answer ALL questions
(10×3=30)
 Answer should not exceed 50 words
 1. Totipotency.
2. Gibberellins.
3. Meristum.
4. Embryo rescue.
5. Synthetic seeds.
6. Transgenics.
7. Polarising.
8. Microtomy.
9. pH.
10. Centrifugation.
 SECTION – B
 Answer ALL questions
(8×5=40)
 Answer should not exceed 250 words
 11. a) Write on sterilization techniques.
(OR)
 b) Comment on auxins.
 12. a) Discuss about cytokinins.
(OR)
 b) Differentiate between dedifferentiation and redifferentiation.
 13. a) Comment on somatic hybridization.
(OR)
 b) Describe callus culture.
 14. a) Comment on microinjection particle bombardment.
(OR)
 b) Describe herbicide resistance.
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 15. a) Define disease resistance.
(OR)
 b) Explain the process of permanent slide preparation.
 16. a) Narrate the materials preparation techniques for electron
microscopy.
(OR)
 b) Write the principle of fluorescence microscopy.
 17. a) Comment on ultra microtomes.
(OR)
 b) Explain TLC.
 18. a) Comment on differential centrifugation.
(OR)
 b) Define density gradient centrifugation.
 SECTION – C
 Answer any THREE questions
(3×10=30)
19. Explain the composition of culture media.
20. Narrate protoplast culture.
21. Give an account on Agrobacterium-mediated gene transfer.
22. Write the principle and application of SEM.
23. Discuss about GLC.
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