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SECTION A(1*2=2 WTAGE)
1.A.WHAT IS THE TEMPERATURE OPTIMA OF TAQ DNA POLYMERASE
B.TEMPERATURE RANGE AT WHICH COHESIVE END LIGATION IS PERFORMED
C.WHICH IS TRANSACTING ELEMENT NEEDED FOR TDNA TRANSFER
D.WHICH IS NOT TELOGEN(ddTTP,ddATP,ddCTP,ddGTP)
2.A.COFACTOR FOR T4 DNA LIGASE
B.A TEMPLATE INDEPENDENT POLYMERASE
C.WHICH AMONG THE FOLLOWING IS tDNA VECTOR
D. WHICH AMONG THE FOLLOWING IS A MARKER GENE USED IN TRANSGENIC PLANTS
SECTION B (ANY 9 )(1*9=9WTAGE)
1.BIOLISTICS
2.TRANSFER DNA
3.VNTR
4.RESTRICTION ENDONUCLEASE
5.PLAQUE LIFT
6.DISARMING
7.AGROBACTERIUM RHIZOGENES
8.LIPOSOMES
9.SYNTENY
10.S35 LABELLING
SECTION C (ANY 4)(2*4=8)
1.BINARY VECTORS
2.AUTOMATED DNA SEQUENCING
3.CLONING OF MITOCHONDRIAL GENES
4.COCULTURING
5.WESTERN BLOTTING
6.MAXAM AND GILBERT SEQUENCING
SECTION D (ANY 2)(2*4=8)
1.CDNA STEPS
2.GENE TRANSFER METHOD FOR TRANSGENIC PLANTS
3.DIFFERENT TEMPERATURE REGIMES IN PCR CYCLES ? WHAT IS THE SIGNIFICANCE OF EACH
4.APPLICATIONS OF DNA FINGERPRINTING
SECTION A(1*2=2 WTAGE)
1.A.WHAT IS THE TEMPERATURE OPTIMA OF TAQ DNA POLYMERASE
B.TEMPERATURE RANGE AT WHICH COHESIVE END LIGATION IS PERFORMED
C.WHICH IS TRANSACTING ELEMENT NEEDED FOR TDNA TRANSFER
D.WHICH IS NOT TELOGEN(ddTTP,ddATP,ddCTP,ddGTP)
2.A.COFACTOR FOR T4 DNA LIGASE
B.A TEMPLATE INDEPENDENT POLYMERASE
C.WHICH AMONG THE FOLLOWING IS tDNA VECTOR
D. WHICH AMONG THE FOLLOWING IS A MARKER GENE USED IN TRANSGENIC PLANTS
SECTION B (ANY 9 )(1*9=9WTAGE)
1.BIOLISTICS
2.TRANSFER DNA
3.VNTR
4.RESTRICTION ENDONUCLEASE
5.PLAQUE LIFT
6.DISARMING
7.AGROBACTERIUM RHIZOGENES
8.LIPOSOMES
9.SYNTENY
10.S35 LABELLING
SECTION C (ANY 4)(2*4=8)
1.BINARY VECTORS
2.AUTOMATED DNA SEQUENCING
3.CLONING OF MITOCHONDRIAL GENES
4.COCULTURING
5.WESTERN BLOTTING
6.MAXAM AND GILBERT SEQUENCING
SECTION D (ANY 2)(2*4=8)
1.CDNA STEPS
2.GENE TRANSFER METHOD FOR TRANSGENIC PLANTS
3.DIFFERENT TEMPERATURE REGIMES IN PCR CYCLES ? WHAT IS THE SIGNIFICANCE OF EACH
4.APPLICATIONS OF DNA FINGERPRINTING
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