University Of Pune Question Paper
Third Year B.Pharmacy Examination, 2010
PHARMACEUTICAL BIOTECHNOLOGY
(2004 Course)
Time : 3 Hours Max. Marks : 80
Instructions:1) Q. No. 1 and 5 are compulsory. Out of the remaining,
attempt 2 questions from Section I and 2 questions from
Section II.
2) Answers to the two Sections should be written in separate
answer books.
3) Neat diagrams must be drawn whenever necessary.
4) Black figures to the right indicate full marks.
SECTION – I
1. Give the principle of r-DNA technology along with significance of enzymes.
Give role of Ti-plasmid in genetic engineering. 10
2. a) Enlist and explain various methods of sereening the recombinants. 10
b) Write a note on Southern blotting techniques and its applications. 5
3. a) What is PTC ? Describe cell suspension culture and its growth parameters in
detail. 10
b) Elaborate role of blood plasma and serum in animal tissue culture. 5
4. Write short notes on (any three) : 15
a) DNA fingerprinting
b) RFLP
c) Meristem culture
d) Primary and established cell lines.
SECTION – II
5. Give details of strain improvement, media, different stages of fermentation and
product recovery in streptomycin production. 10
6. a) Give in detail the method of production of Somatotropin. 10
b) Write about hybridoma technology. 5
7. a) What are the recombinant DNA vaccines ? Elaborate on Hepatitis B
Vaccine. 1 0
b) Give manufacturing of Vit B2 by fermentation process. 5
8. Write short note on any three : 15
a) Waste water management
b) Artificial insemination
c) Applications of enzyme immobilization
d) ELISA.
——————
Third Year B.Pharmacy Examination, 2010
PHARMACEUTICAL BIOTECHNOLOGY
(2004 Course)
Time : 3 Hours Max. Marks : 80
Instructions:1) Q. No. 1 and 5 are compulsory. Out of the remaining,
attempt 2 questions from Section I and 2 questions from
Section II.
2) Answers to the two Sections should be written in separate
answer books.
3) Neat diagrams must be drawn whenever necessary.
4) Black figures to the right indicate full marks.
SECTION – I
1. Give the principle of r-DNA technology along with significance of enzymes.
Give role of Ti-plasmid in genetic engineering. 10
2. a) Enlist and explain various methods of sereening the recombinants. 10
b) Write a note on Southern blotting techniques and its applications. 5
3. a) What is PTC ? Describe cell suspension culture and its growth parameters in
detail. 10
b) Elaborate role of blood plasma and serum in animal tissue culture. 5
4. Write short notes on (any three) : 15
a) DNA fingerprinting
b) RFLP
c) Meristem culture
d) Primary and established cell lines.
SECTION – II
5. Give details of strain improvement, media, different stages of fermentation and
product recovery in streptomycin production. 10
6. a) Give in detail the method of production of Somatotropin. 10
b) Write about hybridoma technology. 5
7. a) What are the recombinant DNA vaccines ? Elaborate on Hepatitis B
Vaccine. 1 0
b) Give manufacturing of Vit B2 by fermentation process. 5
8. Write short note on any three : 15
a) Waste water management
b) Artificial insemination
c) Applications of enzyme immobilization
d) ELISA.
——————
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